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. 2020 Jan;464(1-2):169-180.
doi: 10.1007/s11010-019-03658-w. Epub 2019 Nov 22.

Effect of High Doses of Vitamin D Supplementation on Dengue Virus Replication, Toll-like Receptor Expression, and Cytokine Profiles on Dendritic Cells

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Effect of High Doses of Vitamin D Supplementation on Dengue Virus Replication, Toll-like Receptor Expression, and Cytokine Profiles on Dendritic Cells

Jahnnyer Martínez-Moreno et al. Mol Cell Biochem. .

Abstract

Dengue, caused by dengue virus (DENV) infection, is a public health problem worldwide. Although DENV pathogenesis has not yet been fully elucidated, the inflammatory response is a hallmark feature in severe DENV infection. Although vitamin D (vitD) can promote the innate immune response against virus infection, no studies have evaluated the effects of vitD on DENV infection, dendritic cells (DCs), and inflammatory response regulation. This study aimed to assess the impact of oral vitD supplementation on DENV-2 infection, Toll-like receptor (TLR) expression, and both pro- and anti-inflammatory cytokine production in monocyte-derived DCs (MDDCs). To accomplish this, 20 healthy donors were randomly divided into two groups and received either 1000 or 4000 international units (IU)/day of vitD for 10 days. During pre- and post-vitD supplementation, peripheral blood samples were taken to obtain MDDCs, which were challenged with DENV-2. We found that MDDCs from donors who received 4000 IU/day of vitD were less susceptible to DENV-2 infection than MDDCs from donors who received 1000 IU/day of vitD. Moreover, these cells showed decreased mRNA expression of TLR3, 7, and 9; downregulation of IL-12/IL-8 production; and increased IL-10 secretion in response to DENV-2 infection. In conclusion, the administration of 4000 IU/day of vitD decreased DENV-2 infection. Our findings support a possible role of vitD in improving the innate immune response against DENV. However, further studies are necessary to determine the role of vitD on DENV replication and its innate immune response modulation in MDDCs.

Keywords: Antiviral activity; Double-stranded RNA; Inflammation; Toll-like receptors; Vitamin D receptor.

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References

    1. Immunobiology. 2018 Nov;223(11):608-617 - PubMed

References

    1. Am J Clin Nutr. 1999 May;69(5):842-56 - PubMed

References

    1. Rheumatology (Oxford). 2010 Aug;49(8):1466-71 - PubMed

References

    1. Biochem Biophys Res Commun. 2006 Oct 13;349(1):209-13 - PubMed

References

    1. World J Clin Pediatr. 2017 Feb 8;6(1):1-9 - PubMed

References

    1. J Virol. 2000 Sep;74(17):7814-23 - PubMed

References

    1. PLoS Negl Trop Dis. 2013;7(2):e2060 - PubMed

References

    1. Immunology. 2014 Feb;141(2):143-56 - PubMed

References

    1. J Clin Endocrinol Metab. 2011 Jan;96(1):53-8 - PubMed

References

    1. J Gen Virol. 2017 Dec;98(12):2993-3007 - PubMed

References

    1. PLoS One. 2019 Mar 22;14(3):e0214245 - PubMed

References

    1. Rheum Dis Clin North Am. 2012 Feb;38(1):141-60 - PubMed

References

    1. PLoS Negl Trop Dis. 2017 Oct 11;11(10):e0005904 - PubMed

References

    1. Curr Protoc Immunol. 2012 Nov;Chapter 7:Unit7.32 - PubMed

References

    1. Proc Natl Acad Sci U S A. 2015 Jun 30;112(26):8052-7 - PubMed

References

    1. Hum Immunol. 2012 Nov;73(11):1194-9 - PubMed

References

    1. Cell Microbiol. 2009 Apr;11(4):604-15 - PubMed

References

    1. Nature. 2013 Apr 25;496(7446):504-7 - PubMed

References

    1. J Gen Virol. 2016 Jul;97(7):1584-1591 - PubMed

References

    1. Am J Trop Med Hyg. 2002 Jul;67(1):102-6 - PubMed

References

    1. J Infect Dis. 2007 Jun 15;195(12):1808-17 - PubMed

References

    1. EMBO Rep. 2006 Nov;7(11):1176-81 - PubMed

References

    1. Immunol Invest. 2019 Sep 2;:1-10 - PubMed

References

    1. Lancet. 2015 Jan 31;385(9966):453-65 - PubMed

References

    1. BMC Infect Dis. 2013 Jul 24;13:341 - PubMed

References

    1. Biochim Biophys Acta. 2010 Nov;1803(11):1276-86 - PubMed

References

    1. J Immunol. 2001 Feb 1;166(3):1499-506 - PubMed

References

    1. Exp Hematol. 2000 May;28(5):575-83 - PubMed

References

    1. J Clin Microbiol. 2003 Jun;41(6):2408-16 - PubMed

References

    1. Int J Vasc Med. 2015;2015:917143 - PubMed

References

    1. J Clin Virol. 2011 Mar;50(3):194-200 - PubMed

References

    1. FEMS Immunol Med Microbiol. 2008 Aug;53(3):287-99 - PubMed

References

    1. J Immunol. 2004 Sep 1;173(5):2909-12 - PubMed

References

    1. Osteoporos Int. 2013 May;24(5):1537-53 - PubMed

References

    1. Antiviral Res. 2012 Apr;94(1):57-61 - PubMed

References

    1. Clin Chim Acta. 2018 Mar;478:140-151 - PubMed

References

    1. EMBO Rep. 2018 Aug;19(8): - PubMed

References

    1. Proc West Pharmacol Soc. 2009;52:14-7 - PubMed

References

    1. World J Gastroenterol. 2018 Jan 28;24(4):445-460 - PubMed

References

    1. Clin Dev Immunol. 2013;2013:807971 - PubMed

References

    1. Curr Top Microbiol Immunol. 2006;311:1-16 - PubMed

References

    1. Immunity. 2003 May;18(5):605-17 - PubMed

References

    1. BMJ Open. 2012 Dec 13;2(6):null - PubMed

References

    1. Int Immunopharmacol. 2010 Aug;10(8):922-8 - PubMed

References

    1. Nutrients. 2016 Dec 12;8(12): - PubMed

References

    1. Clin Exp Immunol. 2009 Jul;157(1):48-59 - PubMed

References

    1. Cytokine Growth Factor Rev. 2014 Aug;25(4):415-21 - PubMed

References

    1. Int Immunopharmacol. 2013 Jan;15(1):15-22 - PubMed

References

    1. Eur J Pharmacol. 2017 Feb 5;796:110-114 - PubMed

References

    1. PLoS Negl Trop Dis. 2011 Jan 04;5(1):e926 - PubMed

References

    1. Peptides. 2017 Jun;92:23-30 - PubMed

References

    1. J Immunol. 2006 Nov 15;177(10):7114-21 - PubMed

References

    1. AIDS. 2011 Jun 19;25(10):1305-15 - PubMed

References

    1. Oncotarget. 2017 Nov 20;8(62):106050-106070 - PubMed

References

    1. Nat Rev Immunol. 2007 Aug;7(8):610-21 - PubMed

References

    1. J Clin Microbiol. 2005 Jul;43(7):3267-72 - PubMed

References

    1. Sci Rep. 2016 Jul 26;6:24530 - PubMed

References

    1. Front Immunol. 2014 Dec 17;5:647 - PubMed

References

    1. Clin Exp Immunol. 2016 Apr;184(1):90-100 - PubMed

References

    1. Microbes Infect. 2016 Jul-Aug;18(7-8):510-6 - PubMed

References

    1. PLoS Pathog. 2015 Jul 30;11(7):e1005053 - PubMed

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