Characterization of polysaccharides isolated from Hericium erinaceus and their protective effects on the DON-induced oxidative stress

Int J Biol Macromol. 2020 Jun 1;152:1265-1273. doi: 10.1016/j.ijbiomac.2019.10.223. Epub 2019 Nov 20.


In this study, the structure characteristic of the Hericium erinaceus polysaccharide (HEP) was investigated using Fourier transformed infrared spectrometry (FT-IR), gas chromatography-mass spectrometry (GC-MS), gel permeation chromatography (GPC), methylation and nuclear magnetic resonance (NMR). The results showed that HEP, with a molecular weight of 43 KDa, was mainly composed of glucose and rhamnose. The linkages of the sugar residues of HEP were → 6) β-d-Glcp-(1 → and → 2) -α-l-Rhap-(1 → residue at the end of the branches. The Fusarium toxin deoxynivalenol (DON)-induced cellular injury model for IPEC-J2 cells was established and used to investigate the protective effects of HEP against the oxidative stress. The results suggest that HEP could significantly protect IPEC-J2 cells from DON-induced oxidative stress, inhibit DON-induced apoptosis and reduce the production of reactive oxygen species (ROS). Overall, this study suggested that HEP could be explored as potential antioxidant agents for DON-induced intestinal mucosa injury.

Keywords: Fusarium toxin deoxynivalenol; Oxidative stress; Polysaccharide.

MeSH terms

  • Animals
  • Antioxidants / pharmacology
  • Apoptosis / drug effects
  • Cell Line
  • Cell Survival / drug effects
  • Glucose / metabolism
  • Hericium / chemistry*
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / metabolism
  • Molecular Weight
  • Oxidative Stress / drug effects*
  • Polysaccharides / pharmacology*
  • Protective Agents / pharmacology*
  • Reactive Oxygen Species / metabolism
  • Rhamnose / metabolism
  • Spectroscopy, Fourier Transform Infrared / methods
  • Swine
  • Trichothecenes / pharmacology*


  • Antioxidants
  • Polysaccharides
  • Protective Agents
  • Reactive Oxygen Species
  • Trichothecenes
  • Glucose
  • deoxynivalenol
  • Rhamnose