A novel Hericium erinaceus polysaccharide: Structural characterization and prevention of H 2 O 2-induced oxidative damage in GES-1 cells

Int J Biol Macromol. 2020 Jul 1;154:1460-1470. doi: 10.1016/j.ijbiomac.2019.11.027. Epub 2019 Nov 20.


In this paper, the structural characteristics and bioactivity of a novel polysaccharide from H. erinaceus (HEPN) were investigated, wherein, physico-chemical characterization demonstrated that HEPN with an average molecular weight of 12.713 kDa was composed of mannose (5.13%), glucose (43.02%), and galactose (51.85%). The models in vitro for preventing oxidative damage of human gastric epithelium (GES-1) cells were established and used to investigate the preventive effects of HEPN from oxidative damage. It was found that HEPN could significantly prevent GES-1 cells against H2O2-induced oxidative damage by promoting cell proliferation, inhibiting cell necrosis, reducing ROS levels, regulating mitochondrial membrane potential and maintaining mitochondrial membrane permeability. These results indicated HEPN can effectively prevent gastric cell damage in vitro and suggested the potential application of HEPN as bioactive ingredient for healthy foods.

Keywords: GES-1 cells; Hericium erinaceus polysaccharide; Mitochondrial dysfunction; Oxidative damage; Structural characterization.

MeSH terms

  • Cell Death / drug effects
  • Cell Line
  • Cell Proliferation / drug effects
  • Fungal Polysaccharides / chemistry*
  • Fungal Polysaccharides / pharmacology*
  • Hericium / chemistry*
  • Hydrogen Peroxide / pharmacology*
  • Mitochondria / drug effects
  • Mitochondria / metabolism
  • Oxidative Stress / drug effects*
  • Reactive Oxygen Species / metabolism


  • Fungal Polysaccharides
  • Reactive Oxygen Species
  • Hydrogen Peroxide