Genotyping Neisseria gonorrhoeae gyrA and penA antimicrobial genes from remnant Neisseria gonorrhoeae positive Cepheid Xpert® clinical specimens - A feasibility study

J Microbiol Methods. 2020 Jan;168:105783. doi: 10.1016/j.mimet.2019.105783. Epub 2019 Nov 21.


Neisseria gonorrhoeae (NG) has developed resistance to most antibiotics, making it increasingly difficult to treat. Previous studies have predicted antimicrobial NG susceptibility based on the antimicrobial gene target DNA gyrase subunit A (gyrA) codon serine 91 and the penicillin-binding protein 2 (penA) using Roche Cobas® and Hologic APTIMA™ clinical specimens. We studied whether similar methods could be used on remnant NG-positive Cepheid Xpert® specimens.

Keywords: Antibiotic resistance; Genotyping; Neisseria gonorrhoeae; gyrA; penA.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • DNA Gyrase / genetics*
  • Drug Resistance, Bacterial / genetics*
  • Feasibility Studies
  • Female
  • Genotype
  • Genotyping Techniques
  • Gonorrhea / microbiology
  • Humans
  • Microbial Sensitivity Tests
  • Neisseria gonorrhoeae / classification
  • Neisseria gonorrhoeae / genetics*
  • Pharynx / microbiology
  • Real-Time Polymerase Chain Reaction
  • Rectum / microbiology
  • Serine-Type D-Ala-D-Ala Carboxypeptidase / genetics*
  • Vagina / microbiology


  • Serine-Type D-Ala-D-Ala Carboxypeptidase
  • penicillin-binding protein 2, Neisseria gonorrhoeae
  • DNA Gyrase