Biosynthesis of silver nanoparticles using leaf extract of Aesculus hippocastanum (horse chestnut): Evaluation of their antibacterial, antioxidant and drug release system activities

Mater Sci Eng C Mater Biol Appl. 2020 Feb:107:110207. doi: 10.1016/j.msec.2019.110207. Epub 2019 Oct 30.


Biosynthesis of metal nanoparticles is expected as a cost efficient and ecofriendly option in the research study. Therefore, the aqueous extracts made from leaf of Aesculus hippocastanum (horse chestnut) were used as the reducing factors to synthesize silver nanoparticles (AgNPs) and their antioxidant, antibacterial and the application of resveratrol combined AgNPs as efficient delivery vehicles have also been evaluated. The biosynthesized AgNPs were characterized by UV-Visible Spectroscopy, Fourier-Transform Infrared Spectroscopy (FTIR), X Ray Diffraction (XRD), Zeta potential, and Scanning Electron Microscopy (SEM). The AgNPs were found to be stable at -29.1 mV through zeta potential study. According to the UV-Vis measurements, AgNP formation was observed at a wavelength range of 420-470 nm. The Ag NPs were spherical with a size of 50 ± 5 nm. AgNPs exhibited strong antibacterial activity against all tested bacterial species but have no effect against fungal strains. AgNPs showed an important inhibitive activity counter the DPPH radical and thereby indicate a source for antioxidants. The free radical scavenging activity of AgNP was calculated as 54.72% at the highest concentration (100 ppm). The superoxide radical scavenging activity of AgNPs was found to be increased with increasing concentrations and the average inhibition was about 62.9% as compared to the activity of plant extract. In vitro investigations on the drug delivery from AgNPs exhibited pH dependency; the release was significant (45.6%) below acidic terms (pH 5.2) when in proportion to physiological terms (pH7.4). It was observed that the resveratrol-combined to AgNPs stays on the nanoparticle surface for a lengthy time in the plasma at physiological pH (7.4), so very reducing the flank influence on the normal tissues. It can be concluded that horse chestnut leaves has reduction potential as well as being a capping agent to produce well-defined nanoscale silver particles. Our biosynthesized Ag NPs can be used antibacterial, antioxidant agent and also for as effective delivery vehicles in the near future.

Keywords: Antibacterial activity; Antioxidant activity; Drug delivery system; Green synthesis; Horse chestnut; Silver nanoparticle.

MeSH terms

  • Aesculus / chemistry*
  • Aesculus / metabolism
  • Anti-Bacterial Agents / chemistry*
  • Anti-Bacterial Agents / pharmacology
  • Antioxidants / chemistry*
  • Drug Liberation
  • Gram-Negative Bacteria / drug effects
  • Gram-Positive Bacteria / drug effects
  • Green Chemistry Technology
  • Hydrogen-Ion Concentration
  • Metal Nanoparticles / chemistry*
  • Metal Nanoparticles / toxicity
  • Microbial Sensitivity Tests
  • Particle Size
  • Plant Extracts / chemistry
  • Resveratrol / chemistry
  • Resveratrol / metabolism
  • Silver / chemistry*


  • Anti-Bacterial Agents
  • Antioxidants
  • Plant Extracts
  • Silver
  • Resveratrol