Transnasal Delivery of the Peptide Agonist Specific to Neuromedin-U Receptor 2 to the Brain for the Treatment of Obesity

Mol Pharm. 2020 Jan 6;17(1):32-39. doi: 10.1021/acs.molpharmaceut.9b00571. Epub 2019 Dec 9.

Abstract

Obesity and metabolic syndrome are threats to the health of large population worldwide as they are associated with high mortality, mainly linked to cardiovascular diseases. Recently, CPN-116 (CPN), which is an agonist peptide specific to neuromedin-U receptor 2 (NMUR2) that is expressed predominantly in the brain, has been developed as a new therapeutic candidate for the treatment of obesity and metabolic syndrome. However, treatment with CPN poses a challenge due to the limited delivery of CPN to the brain. Recent studies have clarified that the direct anatomical connection of the nasal cavity with brain allows delivery of several drugs to the brain. In this study, we confirm the nasal cavity as a promising CPN delivery route to the brain for the treatment of obesity and metabolic syndrome. According to the pharmacokinetic study, the clearance of CPN from the blood was very rapid with a half-life of 3 min. In vitro study on its stability in the serum and cerebrospinal fluid (CSF) indicates that CPN was more stable in the CSF than in the blood. The concentration of CPN in the brain was higher after nasal administration, despite its lower concentrations in the plasma than that after intravenous administration. The study on its pharmacological potency suggests the effective suppression of increased body weight in mice in a dose-dependent manner due to the direct activation of NMUR2 by CPN. This results from the higher concentration of corticosterone in blood after nasal administration of CPN as compared to nasal application of saline. In conclusion, the above findings indicate that the nasal cavity is a promising CPN delivery route to the brain to treat obesity and metabolic syndrome.

Keywords: brain delivery; nasal delivery; neuromedin U; nose to brain; obesity; peptide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Intranasal
  • Animals
  • Anti-Obesity Agents / administration & dosage*
  • Anti-Obesity Agents / blood
  • Anti-Obesity Agents / cerebrospinal fluid
  • Anti-Obesity Agents / pharmacokinetics
  • Brain / drug effects*
  • Corticosterone / blood
  • HEK293 Cells
  • Humans
  • Mice
  • Obesity / blood
  • Obesity / cerebrospinal fluid
  • Obesity / drug therapy*
  • Peptides / administration & dosage*
  • Peptides / blood
  • Peptides / cerebrospinal fluid
  • Peptides / pharmacokinetics
  • Rats
  • Rats, Wistar
  • Receptors, Neurotransmitter / agonists*

Substances

  • Anti-Obesity Agents
  • Peptides
  • Receptors, Neurotransmitter
  • neuromedin U receptor
  • Corticosterone