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[Online ahead of print]

Magnetic Resonance Imaging of Mean Cell Size in Human Breast Tumors

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Magnetic Resonance Imaging of Mean Cell Size in Human Breast Tumors

Junzhong Xu et al. Magn Reson Med.

Abstract

Purpose: Cell size is a fundamental characteristic of all tissues, and changes in cell size in cancer reflect tumor status and response to treatments, such as apoptosis and cell-cycle arrest. Unfortunately, cell size can currently be obtained only by pathological evaluation of tumor tissue samples obtained invasively. Previous imaging approaches are limited to preclinical MRI scanners or require relatively long acquisition times that are impractical for clinical imaging. There is a need to develop cell-size imaging for clinical applications.

Methods: We propose a clinically feasible IMPULSED (imaging microstructural parameters using limited spectrally edited diffusion) approach that can characterize mean cell sizes in solid tumors. We report the use of a combination of pulse sequences, using different gradient waveforms implemented on clinical MRI scanners and analytical equations based on these waveforms to analyze diffusion-weighted MRI signals and derive specific microstructural parameters such as cell size. We also describe comprehensive validations of this approach using computer simulations, cell experiments in vitro, and animal experiments in vivo and demonstrate applications in preoperative breast cancer patients.

Results: With fast acquisitions (~7 minutes), IMPULSED can provide high-resolution (1.3 mm in-plane) mapping of mean cell size of human tumors in vivo on clinical 3T MRI scanners. All validations suggest that IMPULSED provides accurate and reliable measurements of mean cell size.

Conclusion: The proposed IMPULSED method can assess cell-size variations in tumors of breast cancer patients, which may have the potential to assess early response to neoadjuvant therapy.

Keywords: IMPULSED; MRI; OGSE; cell size; density; diameter; diffusion; oscillating gradient.

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References

REFERENCES

    1. Kozlowski J, Konarzewski M, Gawelczyk AT. Cell size as a link between noncoding DNA and metabolic rate scaling. Proc Natl Acad Sci U S A. 2003;100:14080-14085.
    1. Baserga R. Is cell size important? Cell Cycle. 2007;6:814-816.
    1. Savage VM, Allen AP, Brown JH, et al. Scaling of number, size, and metabolic rate of cells with body size in mammals. Proc Natl Acad Sci U S A. 2007;104:4718-4723.
    1. Garcia JH, Yoshida Y, Chen H, et al. Progression from ischemic injury to infarct following middle cerebral artery occlusion in the rat. Am. J. Pathol. 1993;142:623-635.
    1. Liang D, Bhatta S, Gerzanich V, Simard JM. Cytotoxic edema: mechanisms of pathological cell swelling. Neurosurg Focus. 2007;22:E2-E2.

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