OsGL6, a conserved AP2 domain protein, promotes leaf trichome initiation in rice

Yunjie Xie; Xiangzhen Yu; Shenfei Jiang; Kaizhuan Xiao; Yupeng Wang; Lele Li; Fuxiang Wang; Wei He; Qiuhua Cai; Huaan Xie; Jianfu Zhang

doi:10.1016/j.bbrc.2019.11.125

OsGL6, a conserved AP2 domain protein, promotes leaf trichome initiation in rice

Biochem Biophys Res Commun. 2020 Feb 5;522(2):448-455. doi: 10.1016/j.bbrc.2019.11.125. Epub 2019 Nov 23.

Authors

Yunjie Xie¹, Xiangzhen Yu¹, Shenfei Jiang¹, Kaizhuan Xiao², Yupeng Wang¹, Lele Li¹, Fuxiang Wang², Wei He², Qiuhua Cai², Huaan Xie³, Jianfu Zhang⁴

Affiliations

¹ College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China; Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350019, China; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan' Crops/Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice in South China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Fuzhou Branch, National Center of Rice Improvement of China/National Engineering Laboratory of Rice/South Base of National Key Laboratory of Hybrid Rice of China, Fuzhou, 350003, China.
² Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350019, China; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan' Crops/Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice in South China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Fuzhou Branch, National Center of Rice Improvement of China/National Engineering Laboratory of Rice/South Base of National Key Laboratory of Hybrid Rice of China, Fuzhou, 350003, China.
³ College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China; Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350019, China; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan' Crops/Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice in South China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Fuzhou Branch, National Center of Rice Improvement of China/National Engineering Laboratory of Rice/South Base of National Key Laboratory of Hybrid Rice of China, Fuzhou, 350003, China. Electronic address: huaanxie@163.com.
⁴ College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China; Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350019, China; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan' Crops/Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice in South China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Fuzhou Branch, National Center of Rice Improvement of China/National Engineering Laboratory of Rice/South Base of National Key Laboratory of Hybrid Rice of China, Fuzhou, 350003, China. Electronic address: jianfzhang@163.com.

PMID: 31767151
DOI: 10.1016/j.bbrc.2019.11.125

Abstract

Trichomes are specialized epidermal cells that play crucial roles in resisting environmental stress and enhancing plant development. In Arabidopsis thaliana, the main genes controlling trichome formation have been consecutively identified. However, few genes like this were reported in rice. In this study, we identified the hairy phenotype of indica variety 75-1-127. This was used to construct a segregation population with a cross of hairless variety Minghui63 (MH63) to fine map the trichome formation genes. Genetic analysis indicated that hairy phenotype was controlled by a pair of dominant genes on chromosome 6, which was designated as GLABRA6 (OsGL6). OsGL6 was an allele of HL6 gene whose sequences containing rich variations in genomes. Compared to wild type, the overexpressing transgenic lines revealed that OsGL6 promoted trichome initiation. We found that OsGL6 interacted with serine/threonine protein kinase OSK3 (OSK3) or COP9 signalosome complex subunit 5a (CSN5) in yeast. These results provide potential information for understanding the molecular mechanism of trichome formation in rice.

Keywords: 75-1-127; Minghui63; OsGL6; Trichome; Trichome initiation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Conserved Sequence*
Gene Expression Regulation, Plant
Organogenesis*
Oryza / genetics
Oryza / growth & development*
Phenotype
Plant Leaves / metabolism
Plant Proteins / chemistry*
Plant Proteins / genetics
Plant Proteins / metabolism*
Protein Binding
Protein Domains
Saccharomyces cerevisiae / metabolism
Subcellular Fractions / metabolism
Trichomes / metabolism

Substances

Plant Proteins