Perturbation of 3-tert-butyl-4-hydroxyanisole in adipogenesis of male mice with normal and high fat diets

Zhendong Sun; Zhi Tang; Xiaoxi Yang; Qian S Liu; Yong Liang; Heidelore Fiedler; Jianqing Zhang; Qunfang Zhou; Guibin Jiang

doi:10.1016/j.scitotenv.2019.135608

Perturbation of 3-tert-butyl-4-hydroxyanisole in adipogenesis of male mice with normal and high fat diets

Sci Total Environ. 2020 Feb 10:703:135608. doi: 10.1016/j.scitotenv.2019.135608. Epub 2019 Nov 19.

Authors

Zhendong Sun¹, Zhi Tang², Xiaoxi Yang³, Qian S Liu³, Yong Liang⁴, Heidelore Fiedler⁵, Jianqing Zhang⁶, Qunfang Zhou⁷, Guibin Jiang¹

Affiliations

¹ State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China.
² Department of POPs Lab, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
³ State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
⁴ Institute of Environment and Health, Jianghan University, Wuhan 430056, China.
⁵ Örebro University, School of Science and Technology, MTM Research Centre, SE-701 82 Örebro, Sweden; UN Environment (UNEP), Chemicals Branch, CH-1219 Châtelaine (GE), Switzerland.
⁶ Department of POPs Lab, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China. Electronic address: jianqingzh@szcdc.net.
⁷ State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China; Institute of Environment and Health, Jianghan University, Wuhan 430056, China. Electronic address: zhouqf@rcees.ac.cn.

PMID: 31767314
DOI: 10.1016/j.scitotenv.2019.135608

Abstract

As one of the widely used anthropogenic food additives, 3-tert-butyl-4-hydroxyanisole (3-BHA) has been found to perturb adipogenesis in vitro and induce lipid accumulation in some strains of oleaginous microalgae. The impact of this chemical on adipocyte development and lipid metabolism in mammals remains to be elucidated. In this study, we performed 18-week oral administration of 3-BHA to male C57BL/6J mice with normal diet (ND) or high-fat diet (HFD) and investigated its impacts on adipogenesis and lipid accumulation in vivo. The results indicated that long-term exposure to 3-BHA impacted the mouse body weight gain, white adipose tissue accumulation, and plasma lipids through transcriptional regulation of adipogenesis, lipid metabolism, and adipocyte endocrine function, while glucose metabolism and insulin sensitivity remained unaffected. HFD-fed mice responded to 3-BHA stimulation differently from ND-fed animals, suggesting potential risks for the human burden of 3-BHA in lean and obese subjects. The findings herein validate 3-BHA as an environmental obesogen, and more caution is recommended for its authorized use as a food antioxidant against lipid rancidity.

Keywords: 3-tert-Butyl-4-hydroxyanisole; Adipogenesis; Food additive; Lipid metabolism; Obesity.

MeSH terms

Adipogenesis / drug effects*
Adipose Tissue, White
Animals
Butylated Hydroxyanisole / toxicity*
Diet, High-Fat
Food Additives / toxicity*
Insulin Resistance
Lipid Metabolism / drug effects
Male
Mice
Mice, Inbred C57BL
Obesity
Weight Gain

Substances

Food Additives
Butylated Hydroxyanisole
3-tert-butyl-4-hydroxyanisole