Aqueous Humor Is Superior to Blood as a Liquid Biopsy for Retinoblastoma

Jesse L Berry; Liya Xu; Ashley Polski; Rima Jubran; Peter Kuhn; Jonathan W Kim; James Hicks

doi:10.1016/j.ophtha.2019.10.026

Aqueous Humor Is Superior to Blood as a Liquid Biopsy for Retinoblastoma

Ophthalmology. 2020 Apr;127(4):552-554. doi: 10.1016/j.ophtha.2019.10.026. Epub 2019 Oct 31.

Authors

Jesse L Berry¹, Liya Xu², Ashley Polski³, Rima Jubran⁴, Peter Kuhn⁵, Jonathan W Kim³, James Hicks⁶

Affiliations

¹ USC Roski Eye Institute, Keck Medical School of the University of Southern California, Los Angeles, California; The Vision Center at Children's Hospital Los Angeles, Los Angeles, California. Electronic address: jesse.berry@med.usc.edu.
² Department of Biological Sciences, Dornsife College of Letters, Arts, and Sciences, University of Southern California, Los Angeles, California.
³ USC Roski Eye Institute, Keck Medical School of the University of Southern California, Los Angeles, California; The Vision Center at Children's Hospital Los Angeles, Los Angeles, California.
⁴ The Children's Center for Cancer and Blood Diseases, Children's Hospital Los Angeles, Los Angeles, California.
⁵ Department of Biological Sciences, Dornsife College of Letters, Arts, and Sciences, University of Southern California, Los Angeles, California; Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, California; Department of Aerospace and Mechanical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, California; Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, California.
⁶ Department of Biological Sciences, Dornsife College of Letters, Arts, and Sciences, University of Southern California, Los Angeles, California; Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, California.

Abstract

Whole genome sequencing was performed on 20 matched blood and aqueous samples; tumor-associated chromosomal changes were found in 0/20 blood vs. 11/20 aqueous samples along with shorter DNA fragments. Thus, aqueous is superior to blood as a liquid biopsy for retinoblastoma.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Aqueous Humor / metabolism*
Cell-Free Nucleic Acids
Child, Preschool
DNA, Neoplasm / genetics
Humans
Infant
Liquid Biopsy
Retinal Neoplasms / blood*
Retinal Neoplasms / diagnosis
Retinoblastoma / blood*
Retinoblastoma / diagnosis
Retinoblastoma Binding Proteins / metabolism*
Ubiquitin-Protein Ligases / metabolism*

Substances

Cell-Free Nucleic Acids
DNA, Neoplasm
RB1 protein, human
Retinoblastoma Binding Proteins
Ubiquitin-Protein Ligases

Abstract

Publication types

MeSH terms

Substances

Grants and funding