The biology of the adenovirus E1B 55K protein

FEBS Lett. 2019 Dec;593(24):3504-3517. doi: 10.1002/1873-3468.13694. Epub 2019 Dec 8.


The adenovirus E1B 55K (E1B) protein plays major roles in productive adenoviral infection and cellular transformation. Interest in E1B increased because of the potential of adenoviruses as therapeutic vectors, and the E1B gene is commonly deleted from adenovirus vectors for anticancer therapy. E1B activities are spatiotemporally regulated through SUMOylation and phosphorylation, and through interactions with multiple partners that occur presumably at different intracellular sites and times postinfection. E1B is implicated in the formation of viral replication compartments and regulates viral genome replication and transcription, transcriptional repression, degradation of cellular proteins, and several intranuclear steps of viral late mRNA biogenesis. Here, we review advances in our understanding of E1B during productive adenovirus replication and discuss fundamental aspects that remain unresolved.

Keywords: DNA replication; E1B 55K; E1B 55kDa; adenovirus; cellular transformation; mRNA export; post-transcriptional processing; protein degradation; replication compartments; viral oncogene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Adenoviridae / metabolism
  • Adenoviridae / physiology*
  • Adenovirus E1B Proteins / chemistry*
  • Adenovirus E1B Proteins / metabolism*
  • Gene Expression Regulation, Viral
  • Models, Molecular
  • Phosphorylation
  • Protein Conformation
  • Sumoylation
  • Virus Replication


  • Adenovirus E1B Proteins