The proteins secreted by human cells (collectively referred to as the secretome) are important not only for the basic understanding of human biology but also for the identification of potential targets for future diagnostics and therapies. Here, we present a comprehensive analysis of proteins predicted to be secreted in human cells, which provides information about their final localization in the human body, including the proteins actively secreted to peripheral blood. The analysis suggests that a large number of the proteins of the secretome are not secreted out of the cell, but instead are retained intracellularly, whereas another large group of proteins were identified that are predicted to be retained locally at the tissue of expression and not secreted into the blood. Proteins detected in the human blood by mass spectrometry-based proteomics and antibody-based immunoassays are also presented with estimates of their concentrations in the blood. The results are presented in an updated version 19 of the Human Protein Atlas in which each gene encoding a secretome protein is annotated to provide an open-access knowledge resource of the human secretome, including body-wide expression data, spatial localization data down to the single-cell and subcellular levels, and data about the presence of proteins that are detectable in the blood.
Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
Secretome protein signature of human pancreatic cancer stem-like cells.J Proteomics. 2016 Mar 16;136:1-12. doi: 10.1016/j.jprot.2016.01.017. Epub 2016 Feb 2. J Proteomics. 2016. PMID: 26850699
The human secretome atlas initiative: implications in health and disease conditions.Biochim Biophys Acta. 2013 Nov;1834(11):2454-61. doi: 10.1016/j.bbapap.2013.04.007. Epub 2013 Apr 19. Biochim Biophys Acta. 2013. PMID: 23603790 Free PMC article.
The cancer secretome, current status and opportunities in the lung, breast and colorectal cancer context.Biochim Biophys Acta. 2013 Nov;1834(11):2242-58. doi: 10.1016/j.bbapap.2013.01.029. Epub 2013 Jan 31. Biochim Biophys Acta. 2013. PMID: 23376433 Review.
Unraveling Mesenchymal Stem Cells' Dynamic Secretome Through Nontargeted Proteomics Profiling.Methods Mol Biol. 2016;1416:521-49. doi: 10.1007/978-1-4939-3584-0_32. Methods Mol Biol. 2016. PMID: 27236694
Bioinformatics tools for secretome analysis.Biochim Biophys Acta. 2013 Nov;1834(11):2442-53. doi: 10.1016/j.bbapap.2013.01.039. Epub 2013 Feb 5. Biochim Biophys Acta. 2013. PMID: 23395702 Review.
Cited by 1 article
Genome-scale reconstructions of the mammalian secretory pathway predict metabolic costs and limitations of protein secretion.Nat Commun. 2020 Jan 2;11(1):68. doi: 10.1038/s41467-019-13867-y. Nat Commun. 2020. PMID: 31896772 Free PMC article.