The multitudinous inositol phosphate family elicits a wide range of molecular effects that regulate countless biological responses. In this review, I provide a methodological viewpoint of the manner in which key advances in the field of inositol phosphate research were made. I also note some of the considerable challenges that still lie ahead.
Keywords: Calcium; HPLC; History; Inhibitors; Inositol; Inositol lipid; Inositol phosphate; Kinases; Mass-assays; Methods; Phosphoinositide; Phospholipase C; Radiolabeling; Signaling.