Protective effect of triterpenes of Ganoderma lucidum on lipopolysaccharide-induced inflammatory responses and acute liver injury

Cytokine. 2020 Mar;127:154917. doi: 10.1016/j.cyto.2019.154917. Epub 2019 Nov 24.


Ganoderma lucidum is a popular medicinal mushroom, which has been used as therapeutic for centuries in traditional Chinese medicine. Although G. lucidum showed strong protective effects in prevention or treatment of a variety of inflammatory diseases, the mechanisms underlying the anti-inflammatory properties of triterpenes of G. lucidum remain undefined. In the current study, we demonstrated that ethanol extract and triterpenes of G. lucidum specifically suppressed LPS-mediated inflammatory responses. Notably, ganodermanontriol inhibited the expressions and interactions of TLR4 and MyD88, NF-κB translocation to nucleus and its DNA binding activity, phosphorylation of p38, ErK1/2 and JNK. In vivo, we showed that ganodermanontriol effectively prevented LPS/D-Galactosamine-induced liver injury by reducing TNF-α and IL-6 production, and decrease of ALT/AST release. Collectively, our results revealed a novel role in inhibition of inflammatory diseases for triterpenes that may act through potential inhibition of TLR4-MyD88-mediated NF-κB and MAPK signaling pathways.

Keywords: Ganoderma lucidum; Inflammation; MAPK; NF-κB; Triterpenes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cell Survival / drug effects
  • Chemical and Drug Induced Liver Injury / etiology
  • Chemical and Drug Induced Liver Injury / metabolism
  • Chemical and Drug Induced Liver Injury / prevention & control*
  • Cytokines / metabolism
  • Female
  • Inflammation / chemically induced
  • Inflammation / prevention & control*
  • Lanosterol / analogs & derivatives*
  • Lanosterol / chemistry
  • Lanosterol / pharmacology
  • Lipopolysaccharides
  • MAP Kinase Signaling System / drug effects
  • Macrophages / cytology
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Mice, Inbred BALB C
  • Molecular Structure
  • NF-kappa B / metabolism
  • Protective Agents / chemistry
  • Protective Agents / pharmacology
  • Reishi / chemistry*
  • Triterpenes / chemistry
  • Triterpenes / pharmacology*


  • Cytokines
  • Lipopolysaccharides
  • NF-kappa B
  • Protective Agents
  • Triterpenes
  • ganodermanontriol
  • Lanosterol