Flagellimonas algicola sp. nov., Isolated from a Marine Red Alga, Asparagopsis taxiformis

Curr Microbiol. 2020 Feb;77(2):294-299. doi: 10.1007/s00284-019-01821-6. Epub 2019 Nov 28.

Abstract

A Gram-stain-negative, strictly aerobic and moderately halophilic bacterium, designated strain AsT0115T, was isolated from a red marine alga, Asparagopsis taxiformis. Cells were non-motile rods showing oxidase-positive and catalase-negative activities. Growth was observed at 15-37 C (optimum, 25 C) and pH 6.5-7.5 (optimum, pH 7.0) and in the presence of 0-11.0% (w/v) NaCl (optimum, 2.0-3.0%). Strain AsT0115T contained iso-C15:0 and iso-C15:1 G as the predominant fatty acids. Menaquinone-6 was identified as the sole isoprenoid quinone. Major polar lipids consisted of phosphatidylethanolamine, an identified aminolipid and three unidentified polar lipids. The G+C content of the genomic DNA calculated from the whole genome sequence was 41.7 mol%. Strain AsT0115T was most closely related to Flagellimonas pacifica sw169T and Flagellimonas flava DSM 22638T with 96.5% and 95.6% 16S rRNA gene sequence similarities, respectively. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AsT0115T formed a phyletic lineage with F. pacifica sw169T within the genus Flagellimonas. Phenotypic, chemotaxonomic, and phylogenetic features clearly suggested that strain AsT0115T represents a novel species of the genus Flagellimonas, for which the name Flagellimonas algicola sp. nov. is proposed. The type strain is AsT0115T (= KACC 19790T = JCM 32942T).

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Flavobacteriaceae / chemistry
  • Flavobacteriaceae / classification*
  • Flavobacteriaceae / genetics*
  • Flavobacteriaceae / isolation & purification
  • Phospholipids / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea
  • Rhodophyta / microbiology*
  • Sequence Analysis, DNA
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • menaquinone 6