Microfluidic Approach for Highly Efficient Viral Transduction

Methods Mol Biol. 2020;2097:55-65. doi: 10.1007/978-1-0716-0203-4_3.

Abstract

Lentiviral vectors enable gene transfer into target cells, but manufacturing is complex, scale-limited, and costly. Here, we describe the use of microfluidic devices for efficient ex vivo gene transfer. Up to four- to fivefold reductions in viral vector usage and two- to fourfold reductions in transduction times can be obtained by using this method.

Keywords: Cell therapy; Gene therapy; Lentiviral vectors; Microfluidics; Retroviral vectors; Transduction; Viral vectors.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adsorption
  • Dimethylpolysiloxanes / chemistry
  • Fibronectins
  • HEK293 Cells
  • Humans
  • Jurkat Cells
  • Microfluidics / methods*
  • Recombinant Proteins
  • Silicon / chemistry
  • Transduction, Genetic*
  • Viruses / metabolism*

Substances

  • Dimethylpolysiloxanes
  • Fibronectins
  • Recombinant Proteins
  • retronectin
  • baysilon
  • Silicon