Lentiviral vectors enable gene transfer into target cells, but manufacturing is complex, scale-limited, and costly. Here, we describe the use of microfluidic devices for efficient ex vivo gene transfer. Up to four- to fivefold reductions in viral vector usage and two- to fourfold reductions in transduction times can be obtained by using this method.
Keywords: Cell therapy; Gene therapy; Lentiviral vectors; Microfluidics; Retroviral vectors; Transduction; Viral vectors.