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. 2020;2097:55-65.
doi: 10.1007/978-1-0716-0203-4_3.

Microfluidic Approach for Highly Efficient Viral Transduction

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Microfluidic Approach for Highly Efficient Viral Transduction

Reginald Tran et al. Methods Mol Biol. .

Abstract

Lentiviral vectors enable gene transfer into target cells, but manufacturing is complex, scale-limited, and costly. Here, we describe the use of microfluidic devices for efficient ex vivo gene transfer. Up to four- to fivefold reductions in viral vector usage and two- to fourfold reductions in transduction times can be obtained by using this method.

Keywords: Cell therapy; Gene therapy; Lentiviral vectors; Microfluidics; Retroviral vectors; Transduction; Viral vectors.

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