Marinobacter fonticola sp. nov., isolated from deep sea cold seep sediment

Abstract

In this study, we report a novel Gram-negative bacterium, designated as strain CS412^T, isolated from deep-sea sediment collected in a cold seep area of the South China Sea. Growth of strain CS412^T occurred at 4-40 °C (optimum, 28 °C), pH 5.0-11.0 (optimum, pH 6.0) and with 0-19 % (w/v) NaCl (optimum, 1-2 %). Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain CS412^T belonged to the genus Marinobacter. The closest phylogenetic neighbours of strain CS412^T were Marinobacter pelagius HS225^T (96.9 %), Marinobacter szutsaonensis NTU-104^T (96.8%), Marinobacter santoriniensis NKSG1^T (96.4%) and Marinobacter koreensisdd-M3^T (96.3 %). The genomic DNA G+C content of strain CS412^T was 58.0 mol%. The principal respiratory quinone was ubiquinone-9 (Q-9). The polar lipids of CS412^T contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, aminophospholipidand and four glycolipids. The major fatty acids of CS412^T contained cyclo-C_{19 : 0}ω8c, C_{16 : 0}, C_{18 : 1}ω7c and C_{18 : 1}ω7c 11-methyl. The results of phylogenetic, physiological, biochemical and morphological analyses suggested that strain CS412^T represents a novel species of the genus Marinobacter, and the name Marinobacter fonticola sp. nov. is proposed with the type species CS412^T (=CCTCC AB 2019197^T=KCTC 72475^T).

Keywords: Marinobacter; cold seep; deep sea.