Whole genome sequencing and phylogenetic characterization of a novel bat-associated picornavirus-like virus with an unusual genome organization

Infect Genet Evol. 2020 Mar;78:104130. doi: 10.1016/j.meegid.2019.104130. Epub 2019 Nov 27.

Abstract

The order Picornavirales is one of the most important viral orders in terms of virus diversity and genome organizations, ranging from a mono- or bi-cistronic expression strategies to the recently described poly-cistronic Polycipiviridae viruses. We report here the description and characterization of a novel picorna-like virus identified in rectal swabs of frugivorous bats in Cambodia that presents an unusual genome organization. Kandabadicivirus presents a unique genome architecture and distant phylogenetic relationship to the proposed Badiciviridae family. These findings highlight a high mosaicism of genome organizations among the Picornavirales.

Keywords: Bats; Nucleotide composition analysis; Phylogeny; Picornavirales; Rectal swabs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Animals
  • Cambodia
  • Capsid Proteins / genetics
  • Chiroptera / virology*
  • Genome, Viral*
  • Open Reading Frames
  • Phylogeny*
  • Picornaviridae / genetics*
  • Picornaviridae / isolation & purification
  • RNA, Viral / chemistry
  • Rectum / virology
  • Whole Genome Sequencing

Substances

  • 3' Untranslated Regions
  • Capsid Proteins
  • RNA, Viral