Multiple Approaches for Enhancing Neural Activity to Promote Neurite Outgrowth of Retinal Explants

Chuan-Chin Chiao; Chin-I Lin; Meng-Jung Lee

doi:10.1007/978-1-0716-0175-4_6

Multiple Approaches for Enhancing Neural Activity to Promote Neurite Outgrowth of Retinal Explants

Methods Mol Biol. 2020:2092:65-75. doi: 10.1007/978-1-0716-0175-4_6.

Authors

Chuan-Chin Chiao¹, Chin-I Lin², Meng-Jung Lee^{3

4

5}

Affiliations

¹ Institute of Systems Neuroscience, National Tsing Hua University, Hsinchu, Taiwan. ccchiao@life.nthu.edu.tw.
² Institute of Systems Neuroscience, National Tsing Hua University, Hsinchu, Taiwan.
³ Natural and Medical Sciences Institute, University of Tübingen, Reutlingen, Germany.
⁴ Multi Channel Systems GmbH, Reutlingen, Germany.
⁵ Graduate Training Centre of Neuroscience, University of Tübingen, Tübingen, Germany.

PMID: 31786782
DOI: 10.1007/978-1-0716-0175-4_6

Abstract

Activity is important for neural development and regeneration. Enhancing neural activity can facilitate axon regrowth of retinal ganglion cells. Here, we describe various methods, including electrical stimulation, pharmacological manipulation, and optogenetics, to elevate neural activity of retinal explants in mice and to analyze their effects on promoting neurite outgrowth in organotypic culture.

Keywords: Axon regeneration; Electrical stimulation; Optogenetics; Organotypic culture; Retinal ganglion cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Axons / physiology
Electric Stimulation / methods
Female
Male
Mice
Mice, Inbred C57BL
Nerve Regeneration / physiology
Neurites / physiology*
Neurogenesis / physiology
Neuronal Outgrowth / physiology*
Optogenetics / methods
Retina / physiology*
Retinal Ganglion Cells / physiology*