Isolation and structural determination of rat neuromedin U

Biochem Biophys Res Commun. 1988 Oct 14;156(1):355-60. doi: 10.1016/s0006-291x(88)80848-9.


Rat neuromedin U was isolated from the small intestine using mainly immunoaffinity chromatography and radioimmunoassay for pig neuromedin U-8. The amino acid sequence of rat neuromedin U was determined by microsequence analysis to be Tyr-Lys-Val-Asn-Glu-Tyr-Gln-Gly-Pro-Val-Ala-Pro-Ser-Gly-Gly- Phe-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2, and this structure was confirmed by synthesis. Although the C-terminal heptapeptide amide structure of pig neuromedin U is completely conserved in rat neuromedin U, the remainder of the peptide reveals nine amino acid replacements and two amino acid deletions when compared to pig neuromedin U-25. Rat neuromedin U exerts two-fold potent uterus stimulant activity as compared to pig neuromedin U-25.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromatography, Affinity
  • Female
  • Intestine, Small / analysis*
  • Male
  • Molecular Sequence Data
  • Neuropeptides / chemical synthesis
  • Neuropeptides / isolation & purification*
  • Neuropeptides / physiology
  • Radioimmunoassay
  • Rats
  • Rats, Inbred Strains
  • Species Specificity
  • Swine
  • Uterine Contraction


  • Neuropeptides
  • neuromedin U 8