Baicalin suppress growth and virulence-related factors of methicillin-resistant Staphylococcus aureus in vitro and vivo

Microb Pathog. 2020 Feb;139:103899. doi: 10.1016/j.micpath.2019.103899. Epub 2019 Nov 29.

Abstract

A Staphylococcus aureus (S.aureus) was isolated from pigs suffered in pneumonia that can't be cured by antibiotic such as methicillin and vancomycin. It was demonstrated that baicalin, an active natural compound extracted from the traditional Chinese medicinal, possess antimicrobial activity. In the present study, we evaluate it efficacy in vitro and vivo against this isolated methicillin-resistant S.aureus (MRSA). Our findings demonstrated that baicalin can inhibit S. aureus growth in a dose-dependent manner and attenuate the biofilm formation. Scanning electron microscopies showed that cell membrane was damaged and accompany with contents leaks after treated with high concentration of baicalin. In addition, baicalin exerted inhibitory effects on the expression of S.aureus virulence-related factors. Moreover, baicalin treated mice had enhanced survival after a lethal dose of S.aureus infection compared with untreated mice. Simultaneously, the pathological tissue damage and bacterium burden were decrease in baicalin treated mice. These data demonstrated that baicalin displayed a high effectiveness in vitro and vivo against MRSA infection, suggesting that baicalin may potentially be used to treat MRSA infection.

Keywords: Baicalin; Methicillin-resistant; Resistance; Staphylococcuss aureus; Virulence-related factor.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Drugs, Chinese Herbal / administration & dosage*
  • Female
  • Flavonoids / administration & dosage*
  • Gene Expression Regulation, Bacterial / drug effects
  • Methicillin-Resistant Staphylococcus aureus / drug effects*
  • Methicillin-Resistant Staphylococcus aureus / genetics
  • Methicillin-Resistant Staphylococcus aureus / growth & development
  • Methicillin-Resistant Staphylococcus aureus / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Staphylococcal Infections / drug therapy
  • Staphylococcal Infections / microbiology
  • Staphylococcal Infections / veterinary*
  • Swine
  • Swine Diseases / drug therapy*
  • Swine Diseases / microbiology
  • Virulence Factors / genetics*
  • Virulence Factors / metabolism

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Drugs, Chinese Herbal
  • Flavonoids
  • Virulence Factors
  • baicalin