Selective suppression of oral allergen-induced anaphylaxis by Allergin-1 on basophils in mice

Int Immunol. 2020 Mar 7;32(3):213-219. doi: 10.1093/intimm/dxz075.


Mast cells (MCs) play a critical role in oral allergen-induced anaphylaxis. However, the contribution of basophils to the anaphylaxis remains unclear. The inhibitory immunoreceptor Allergin-1 is highly expressed on MCs and basophils and inhibits FcεRI-mediated signaling in MCs. Here, we show that Allergin-1-deficient (Milr1-/-) mice developed more severe hypothermia, a higher mortality rate and a greater incidence of diarrhea than did wild-type (WT) mice in an oral ovalbumin (OVA)-induced food allergy model. MC-deficient Mas-TRECK mice, which had been reconstituted with either WT or Milr1-/- bone marrow-derived cultured MCs, did not develop hypothermia in this food allergy model. On the other hand, depletion of basophils by injection of anti-CD200R3 antibody rescued Milr1-/- mice from lethal hypothermia but not from diarrhea. In vitro analyses demonstrated that Allergin-1 inhibits IgE-dependent activation of both human and mouse basophils. Thus, Allergin-1 on basophils selectively suppresses oral allergen-induced anaphylaxis.

Keywords: diarrhea; food allergy; mast cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaphylaxis / immunology*
  • Animals
  • Basophils / immunology*
  • Disease Models, Animal
  • Female
  • Food Hypersensitivity / immunology
  • Immunoglobulin E / immunology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout
  • Receptors, Immunologic / administration & dosage
  • Receptors, Immunologic / deficiency
  • Receptors, Immunologic / immunology*


  • Milr1 protein, mouse
  • Receptors, Immunologic
  • Immunoglobulin E