Expression analysis of genes encoding malectin-like domain (MLD)- and leucine-rich repeat (LRR)- containing proteins in Arabidopsis thaliana

Biosci Biotechnol Biochem. 2020 Jan;84(1):154-158. doi: 10.1080/09168451.2019.1661769. Epub 2019 Sep 5.

Abstract

Malectin is a maltose-binding endoplasmic reticulum protein conserved in animals. In Arabidopsis thaliana, we identified four genes that encode malectin-like domain (MLD)- and leucine-rich repeat (LRR)-containing proteins (AtMLLRs): two were receptor-like proteins (AtMLLR1 and 2) and the other two were extracellular proteins (AtMLLR3 and 4). The promoter:G3GFP+promoter:GUS assay indicated the organ- and cell-specific expression of the AtMLLR2 and AtMLLR3 genes.Abbreviations: Cmr: chloramphenicol-resistance marker; G3GFP: G3 green fluorescent protein; GUS: β-glucuronidase; KD: kinase domain; LRR: leucine-rich repeat; MLD: malectin-like domain; RLK: receptor-like kinase; SP: signal peptide; TMD: transmembrane domain; Tnos: nopaline synthase terminator.

Keywords: Arabidopsis thaliana; Gateway cloning; leucine-rich repeat; malectin-like domain.

MeSH terms

  • Arabidopsis / genetics*
  • Arabidopsis Proteins / genetics*
  • Endoplasmic Reticulum / metabolism
  • Gene Expression*
  • Glucuronidase / chemistry
  • Green Fluorescent Proteins / chemistry
  • Lectins / genetics*
  • Leucine / genetics
  • Leucine-Rich Repeat Proteins
  • Membrane Proteins / genetics*
  • Microscopy, Fluorescence
  • Phylogeny
  • Plants, Genetically Modified
  • Protein Domains / genetics
  • Proteins / genetics*
  • Staining and Labeling

Substances

  • Arabidopsis Proteins
  • Lectins
  • Leucine-Rich Repeat Proteins
  • Membrane Proteins
  • Proteins
  • Green Fluorescent Proteins
  • Glucuronidase
  • Leucine