Background: Gingiva-derived mesenchymal stem cells (GMSCs) obtained multipotent differentiation and immunomodulatory properties. However, collecting healthy gingival tissues may be challenging in the clinical situation. Thus, in our present study, we aim to evaluate whether the immunomodulatory capacity of gingiva-derived mesenchymal stem cells from inflamed gingival tissues (iGMSCs) is impaired and find a way to rescue their deficient properties.
Methods: We compared the immunomodulation capacity of GMSCs and iGMSCs using an in vitro co-culture system and a mouse colitis model. T cell apoptosis, T helper 17 (Th17), and regulatory T (Treg) cell differentiation were detected by flow cytometry analysis.
Results: We demonstrated that iGMSCs obtained a decreased immunomodulatory capacity compared with GMSCs. Acetylsalicylic acid (ASA) pretreatment was able to rescue iGMSCs' impaired immunomodulatory properties. Mechanistically, ASA was capable of upregulating the expression of Fas ligand (FasL) in iGMSCs, leading to an improvement in iGMSC-mediated T cell apoptosis and therapeutic efficacy in the treatment in colitis mice.
Conclusions: This study indicates that the deficient immunomodulatory function of iGMSCs could be rescued by ASA pretreatment via upregulating of FasL in mice. This strategy might serve as a practical approach to rescue deficient MSC function for further therapeutic application.
Keywords: Cell therapy; Fas pathway; Immunomodulation; Mesenchymal stem cell.
Conflict of interest statement
The authors declare that they have no competing interests.
Hydrogen Sulfide Promotes Immunomodulation of Gingiva-Derived Mesenchymal Stem Cells via the Fas/FasL Coupling PathwayR Yang et al. Stem Cell Res Ther 9 (1), 62. PMID 29523215.These findings showed that H2S was required to maintain immunomodulation of GMSCs, which was mediated by Fas/FasL coupling-induced T-cell apoptosis.
[Acetylsalicylic Acid Treatment Enhanced Immunomodulatory Function of Mesenchymal Stem Cells Derived From Gingiva]RL Yang et al. Beijing Da Xue Xue Bao Yi Xue Ban 49 (5), 872-877. PMID 29045972.ASA enhanced immunomodulation of GMSCs and increased the capacity of GMSCs to ameliorate DSS-induced colitis in mice.
Gingivae Contain Neural-Crest- And Mesoderm-Derived Mesenchymal Stem CellsX Xu et al. J Dent Res 92 (9), 825-32. PMID 23867762.Gingivae represent a unique soft tissue that serves as a biological barrier to cover the oral cavity side of the maxilla and mandible. Recently, the gingivae were identif …
Updates on GMSCs Treatment for Autoimmune DiseasesF Huang et al. Curr Stem Cell Res Ther 13 (5), 345-349. PMID 29468980. - ReviewThe availability of gingival mesenchymal stem cells (GMSCs), together with their potent capacity of multi-directional differentiation and inflammatory modulation, making …
Human Oral Mucosa and Gingiva: A Unique Reservoir for Mesenchymal Stem CellsQZ Zhang et al. J Dent Res 91 (11), 1011-8. PMID 22988012. - ReviewMesenchymal stem cells (MSCs) represent a heterogeneous population of progenitor cells with self-renewal and multipotent differentiation potential. Aside from their regen …
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- 81571815/National Science Foundation of China
- 81600865/National Science Foundation of China
- 81300850/National Science Foundation of China
- 7152156/the Beijing Municipal Natural Science Foundation
- No.20110203, No.20170109/Young Scientists Fund of PKUSS
- PKUSSNCT-11A07/the New Clinical Technology Fund of PKUSS
- BMU2018MX007/the Peking University Medicine Seed Fund for Interdisciplinary Research
- BMU2018PYB009/the Peking University Medicine Fund of Fostering Young Scholars' Scientific & Technological Innovation
- 51903003/National Natural Science Foundation of China
- 81970909/National Natural Science Foundation of China