Genetic mapping of ionomic quantitative trait loci in rice grain and straw reveals OsMOT1;1 as the putative causal gene for a molybdenum QTL qMo8

Mol Genet Genomics. 2020 Mar;295(2):391-407. doi: 10.1007/s00438-019-01632-1. Epub 2019 Dec 3.

Abstract

Rice is a major dietary source of essential mineral nutrients and toxic elements (aka ionome) for humans. However, the genetic basis underlying the variation in ionome is still largely unknown. Here, we mapped 51 and 61 quantitative trait loci (QTLs) controlling the concentrations of 13 and 15 elements in rice (Oryza sativa L.) grain and straw, respectively, using a recombinant inbred lines (RILs) that were grown at three different field sites in 3 years. Several QTLs were repeatedly detected in both grain and straw or in multiple years; the resulting 87 unique QTLs with 17 of them (20%) were co-localized with previously reported corresponding QTLs and 70 were novel ionomic QTLs. At least, 14 genomic clusters that controlled the concentrations of multiple elements were identified. Furthermore, we identified a molybdate transporter gene OsMOT1;1 as the putative causal gene for a QTL controlling molybdenum concentration in both straw and grain. QTL analyses based on the concentrations of multiple elements in both grain and straw of RIL population grown in three field sites in 3 years allow us to identify tissue common QTLs and reproducible QTLs that were validated in multiple years. The identification of ionomic QTLs will be useful in revealing the molecular mechanisms underlying the accumulation of elements in rice and providing the opportunity to reduce the accumulation of toxic elements and enrich the accumulation of beneficial elements in rice grain.

Keywords: Arsenic; Cadmium; Ionome; Molybdenum; Quantitative trait locus; RICE.

MeSH terms

  • Anion Transport Proteins / genetics*
  • Anion Transport Proteins / metabolism
  • Chromosome Mapping
  • Chromosomes, Plant / genetics
  • Edible Grain / genetics
  • Edible Grain / metabolism
  • Molybdenum / metabolism*
  • Oryza / genetics*
  • Oryza / metabolism
  • Quantitative Trait Loci / genetics*

Substances

  • Anion Transport Proteins
  • molybdate
  • Molybdenum