Serum lipidome analysis of healthy beagle dogs receiving different diets

Metabolomics. 2019 Dec 3;16(1):1. doi: 10.1007/s11306-019-1621-3.


Introduction: Food and dietary ingredients have significant effects on metabolism and health.

Objective: To evaluate whether and how different diets affected the serum lipidomic profile of dogs.

Methods: Sixteen healthy beagles were fed a commercial dry diet for 3 months (control diet). After an overnight fasting period, a blood sample was taken for serum lipidomic profile analysis, and each dog was then randomly assigned to one of two groups. Group 1 was fed a commercial diet (Diet 1) and group 2 was fed a self-made, balanced diet supplemented with linseed oil and salmon oil (Diet 2) for 3 months. After an overnight fasting period, a blood sample was taken from each dog. Serum cholesterol and triacylglycerol analyses were performed and the serum lipidomic profiles were analyzed using targeted liquid chromatography-mass spectrometry.

Results: Dogs fed the supplemented self-made diet (Diet 2) had significantly higher omega-3 fatty acid-containing lipids species and significantly lower saturated and mono- and di-unsaturated lipid species. Concentrations of sphingosine 1-phosphate species S1P d16:1 and S1P d17:1 were significantly increased after feeding Diet 2.

Conclusion: This study found that different diets had significant effects on the dog's serum lipidomic profile. Therefore, in studies that include lipidomic analyses, diet should be included as a confounding factor.

Keywords: Canine; Fatty acids; Lipid metabolism; Mass spectrometry; PUFA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cholesterol / blood
  • Chromatography, High Pressure Liquid
  • Diet* / veterinary
  • Dogs
  • Fish Oils / administration & dosage
  • Linseed Oil / administration & dosage
  • Lipids / blood*
  • Lysophospholipids / blood
  • Male
  • Mass Spectrometry
  • Principal Component Analysis
  • Sphingosine / analogs & derivatives
  • Sphingosine / blood
  • Triglycerides / blood


  • Fish Oils
  • Lipids
  • Lysophospholipids
  • Triglycerides
  • sphingosine 1-phosphate
  • salmon oil
  • Linseed Oil
  • Cholesterol
  • Sphingosine