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. 2019 Nov 11:24:e00398.
doi: 10.1016/j.btre.2019.e00398. eCollection 2019 Dec.

Genome-Wide identification of doublesex and Mab-3-Related transcription factor (DMRT) genes in nile tilapia ( oreochromis niloticus)

Imran Zafar  1 , Mohd Ashraf Rather  2 , Bhushan C Dhandare  2
Affiliations
Free PMC article

Genome-Wide identification of doublesex and Mab-3-Related transcription factor (DMRT) genes in nile tilapia ( oreochromis niloticus)

Imran Zafar et al. Biotechnol Rep (Amst). .
Free PMC article

Abstract

Doublesex and Mab-3-related transcription factor (DMRT) gene family is extensively known for its contribution in sex determination and differentiation across phyla. Here we report the identification of five DM (doublesex and mab-3) domain genes in the Nile tilapia which includes DMRT1, DMRTa2, DMRT2a, DMRT2b and DMRT3a. The full-length sequence of DMRT genes ranges from 3526 (DMRTA2) to 1471bp (DMRT1) which encode putative proteins series from 469 to 372 amino acids. All the DMRT proteins contained at least one conserved DNA-binding DM domain. Sub-cellular localization and gene ontology revealed DMRT1 protein is maximum localized in nuclear region and gene ontology analysis showed the molecular function of 48.2%, biological process 43.6% and cellular component 25%. Chromosomal location and synteny analysis displayed that DMRT genes mostly cluster linkage group 12. Altogether, our findings provide vital genomic information for future studies of biochemical, physiological, and phylogenetic studies on DMRT genes in teleost.

Keywords: DMRT; Genes; Nile tilapia; Sex determination.

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Conflict of interest statement

All authors reported that there is no conflict of interest related with this work.

Figures

Fig. 1
Fig. 1
DMRT proteins annotation of Nile tilapia.
Fig. 2
Fig. 2
The conserved DM domain of all DMRT members.
Fig. 3
Fig. 3
A motif blocks of all DMRT protein of Nile tilapia with other vertebrates.
Fig. 4
Fig. 4
Protein DMRT1 interaction (Protein-protein interaction) with other proteins.
Fig. 5
Fig. 5
A localization probability of DMRT1 in Nile tilapia.
Fig. 6
Fig. 6
Protein DMRT1 and DNA interaction (Protein-DNA interaction).
Fig. 7
Fig. 7
Neighbor-joining-based phylogenetic tree of DMRT proteins sequences of Nile tilapsia with other vertebrates.
Fig. 8
Fig. 8
Analysis of conserved synteny blocks harboring DMRT genes in several vertebrates.
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