The sqt-1 gene of C. elegans encodes a collagen critical for organismal morphogenesis

Cell. 1988 Nov 18;55(4):555-65. doi: 10.1016/0092-8674(88)90214-0.

Abstract

Different mutations in the sqt-1 gene of C. elegans can lengthen, shorten, or helically twist the entire animal. We have cloned the sqt-1 gene and have shown that it encodes a collagen. sqt-1 was localized to a 35 kb region of DNA by physical mapping of chromosomal deficiencies. A transposon (Tc1)-induced mutation of sqt-1 was generated and utilized to identify the sqt-1 gene within this 35 kb region. Sequence analysis of the sqt-1 gene shows that it encodes a 32 kd collagen polypeptide that is similar in size and structure to other members of the C. elegans collagen family. The Tc1 insertion mutant has no detectable sqt-1 transcripts, yet it is morphologically normal, indicating that the null phenotype of sqt-1 is wild type. These results demonstrate that collagen mutations can have dramatic effects on organismal morphology.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Caenorhabditis / genetics*
  • Caenorhabditis / growth & development
  • Collagen / genetics*
  • Electrophoresis, Polyacrylamide Gel
  • Genes*
  • Molecular Sequence Data
  • Morphogenesis*
  • Mutation
  • Transcription, Genetic

Substances

  • Collagen

Associated data

  • GENBANK/J03146