Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 18 (6), 6801-6806

Upregulation of MANCR Predicts Poor Survival in Patients With Gastric Cancer

Affiliations

Upregulation of MANCR Predicts Poor Survival in Patients With Gastric Cancer

Ling Yao et al. Oncol Lett.

Abstract

A previous study revealed that MANCR (mitotically associated long non-coding RNA) is implicated in breast cancer. The present study investigated the potential role of MANCR in gastric cancer (GC) and revealed that MANCR was upregulated in GC tissues compared with non-cancerous tissues. MANCR expression was not affected by clinical stages and a high MANCR expression level was associated with poor survival time. MicroRNA (miR)-101 was downregulated in cancer tissues compared with non-cancerous tissues and was inversely associated with MANCR expression. MANCR overexpression in GC cell lines in vitro resulted in miR-101 downregulation; however, miR-101 overexpression did not alter MANCR expression. Furthermore, MANCR overexpression promoted, while miR-101 overexpression inhibited GC cell proliferation. In addition, miR-101 overexpression decreased the effect of MANCR overexpression. The results obtained in the present study revealed that MANCR expression was associated with the survival of patients with GC, and MANCR overexpression in vitro may promote GC by downregulating miR-101 and increasing the proliferation of GC cells.

Keywords: gastric cancer; microRNA-101; mitotically associated long non-coding RNA.

Figures

Figure 1.
Figure 1.
MANCR was upregulated in patients with GC but not affected by clinical stages. (A) Analysis of MANCR expression data using the paired t-test revealed that MANCR was significantly upregulated in GC tissues compared with adjacent non-cancerous tissues. (B) The one-way ANOVA and Tukey post hoc test revealed no significant differences in the expression level of MANCR in GC tissues in patients with different clinical stages. *P<0.05. MANCR, mitotically associated long non-coding RNA; GC, gastric cancer.
Figure 2.
Figure 2.
Patients with a high MANCR expression level exhibited a poor prognosis. Survival curve analysis revealed that patients with high MANCR expression had a significantly worse overall survival rate compared with patients with low expression. MANCR, mitotically associated long non-coding RNA.
Figure 3.
Figure 3.
miR-101 expression was inversely associated with MANCR expression. (A) Analysis of reverse-transcription quantitative polymerase chain reaction data revealed that miR-101 was significantly downregulated in GC tissues compared with adjacent non-cancerous tissues in GC patients. Linear regression revealed that miR-101 was significantly and inversely associated with MANCR (B) in GC tissues but (C) not in healthy non-cancerous tissues. *P<0.05. miR, microRNA; MANCR, mitotically associated long non-coding RNA; GC, gastric cancer.
Figure 4.
Figure 4.
miR-101 expression was regulated by MANCR in GC cells. The one-way ANOVA and Tukey post hoc test revealed that expression levels of (A) MANCR and (B) miR-101 were significantly increased 24 h following transfection compared with the C and NC groups. (C) MANCR overexpression resulted in miR-101 downregulation in GC cells compared with the C and NC groups. (D) miR-101 overexpression did not alter MANCR expression compared with the C and NC groups. (E) MANCR siRNA mediated the upregulation of miR-101 compared with the C and NC groups. *P<0.05. miR, microRNA; MANCR, mitotically associated long-non coding RNA; GC, gastric cancer; C, control; NC, negative control; si, small interfering.
Figure 5.
Figure 5.
MANCR regulates GC cell proliferation through miR-101. Analysis of the Cell Counting Kit-8 data by the one-way ANOVA and Tukey post hoc test revealed that, compared with the C and NC groups, MANCR overexpression promoted, while miR-101 overexpression inhibited GC cell proliferation. Additionally, miR-101 overexpression decreased the effect of MANCR overexpression on cell proliferation. *P<0.05. MANCR, mitotically associated long non-coding RNA; GC, gastric cancer; miR, microRNA; C, control; NC, negative control.

Similar articles

See all similar articles

LinkOut - more resources

Feedback