Draft genome sequence reveals co-occurrence of multiple antimicrobial resistance and plant probiotic traits in rice root endophytic strain Burkholderia sp. LS-044 affiliated to Burkholderia cepacia complex

Asif Hameed; Mariyam Shahina; Wei-An Lai; Paul Stothard; Li-Sen Young; Shih-Yao Lin; Chiu-Chung Young

doi:10.1016/j.jgar.2019.11.017

Draft genome sequence reveals co-occurrence of multiple antimicrobial resistance and plant probiotic traits in rice root endophytic strain Burkholderia sp. LS-044 affiliated to Burkholderia cepacia complex

J Glob Antimicrob Resist. 2020 Mar:20:28-30. doi: 10.1016/j.jgar.2019.11.017. Epub 2019 Dec 3.

Authors

Asif Hameed¹, Mariyam Shahina¹, Wei-An Lai¹, Paul Stothard², Li-Sen Young³, Shih-Yao Lin¹, Chiu-Chung Young⁴

Affiliations

¹ Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, 145, XingDa Rd., Taichung, 40227, Taiwan.
² Department of Agricultural, Food and Nutritional Science, University of Alberta, 1427 College Plaza, Edmonton, Alberta, Canada.
³ Tetanti AgriBiotech Inc., No. 1 Gongyequ 10th Road, Xitun District, Taichung City 40755, Taiwan.
⁴ Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, 145, XingDa Rd., Taichung, 40227, Taiwan; Innovation and Development Center of Sustainable Agriculture, National Chung Hsing University, Taichung 402, Taiwan. Electronic address: ccyoung@mail.nchu.edu.tw.

PMID: 31809939
DOI: 10.1016/j.jgar.2019.11.017

Abstract

Objectives: Members of the Burkholderia cepacia complex (Bcc) have been isolated from various environmental and clinical samples and reportedly pose a threat to human health. Here we examine the draft genome sequence of Burkholderia sp. LS-044, an antibiotic-resistant endophytic strain affiliated to the Bcc (ST895) inhabiting rice (Oryza sativa ssp. japonica cv. Tainung 71) root.

Methods: Antimicrobial susceptibility of LS-044 was evaluated comparatively with other Burkholderia sp. (CC-Al74 and CC-3XP9) using commercial ATB PSE 5 test strips. The genome of LS-044 was sequenced using an Illumina MiSeq platform. Plant probiotic and antimicrobial resistance genes were screened by Rapid Annotation using Subsystem Technology (RAST), CARD 2017, NCBI and/or UniProt.

Results: Plant-associated members of Bcc (LS-044 and CC-Al74) exhibited greater resistance to the majority of antibiotics tested. The draft genome sequence of LS-044 contained 8.78 Mbp in 62 contigs having a G + C content of 66.5%, 8868 coding sequences and 75 RNAs. The genome harboured genes coding for LysR-type β-lactamase transcription regulator, classes A, C and D β-lactamases, several metal-dependent β-lactamases, antibiotic efflux proteins, and proteins conferring resistance to colistin, streptothricin, colicin and fluoroquinolones. Similarly, it also possessed genes for copper homeostasis, copper-cobalt-zinc-cadmium-chromium resistance and reduction of mercury. Genes involved in flagellar motility, hydrolysis of murein and chitin, production of siderophore and auxin, and metabolism of aromatic compounds were also found.

Conclusion: Genome sequence data revealed an interlinked occurrence of plant probiotic traits and antimicrobial resistance in the rice root endophyte LS-044.

Keywords: Antifungal; Multilocus sequence typing; Plant probiotic; Streptothricin; β-Lactamase.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Base Composition
Burkholderia / classification*
Burkholderia / genetics
Burkholderia / isolation & purification
Drug Resistance, Multiple, Bacterial*
Genome Size
Genome, Bacterial
High-Throughput Nucleotide Sequencing
Humans
Molecular Sequence Annotation
Oryza / microbiology*
Plant Roots / microbiology
Probiotics
Whole Genome Sequencing / methods*