Gallidermin: A New Lanthionine-Containing Polypeptide Antibiotic

Eur J Biochem. 1988 Oct 15;177(1):53-9. doi: 10.1111/j.1432-1033.1988.tb14344.x.

Abstract

Gallidermin is a new member of the class of lanthionine-containing peptide antibiotics, which are summarized under the common name lantibiotics. The lantibiotic gallidermin is produced by Staphylococcus gallinarum (F16/P57) Tü3928, and it exhibits activities against the Propionibacteria, involved in acne disease. Gallidermin differs from the recently discovered tetracyclic 21-residue peptide antibiotic epidermin only in a Leu/Ile exchange in position 6. The isolation procedures for gallidermin included adsorption directly from the culture broth, ion-exchange chromatography of the amphiphilic and basic polypeptide followed by desalting, and final purification by reversed-phase HPLC. The structural elucidation of the polypeptide containing four thioether bridges involved mainly a combination of automated gas-phase sequencing, thermospray liquid chromatography/mass spectrometry and fast-atom-bombardment mass spectrometry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine / analogs & derivatives*
  • Alanine / analysis
  • Amino Acid Sequence
  • Anti-Bacterial Agents* / isolation & purification*
  • Anti-Bacterial Agents* / pharmacology
  • Bacteriocins
  • Chromatography, High Pressure Liquid
  • Chromatography, Ion Exchange
  • Gas Chromatography-Mass Spectrometry
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Peptides*
  • Peptides, Cyclic / isolation & purification
  • Peptides, Cyclic / pharmacology
  • Staphylococcus / metabolism
  • Sulfides

Substances

  • Anti-Bacterial Agents
  • Bacteriocins
  • Peptides
  • Peptides, Cyclic
  • Sulfides
  • gallidermin
  • epidermin
  • lanthionine
  • Alanine