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, 12, 10129-10138
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Propofol Suppresses Proliferation, Migration, Invasion And Promotes Apoptosis By Upregulating microRNA-140-5p In Gastric Cancer Cells

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Propofol Suppresses Proliferation, Migration, Invasion And Promotes Apoptosis By Upregulating microRNA-140-5p In Gastric Cancer Cells

Fengbo Zhu et al. Onco Targets Ther.

Abstract

Purpose: This study aimed to investigate the anti-tumor effect of propofol on gastric cancer (GC) and its underlying mechanisms.

Patients and methods: SGC-7901 and MKN45 cells were transfected and divided into the following groups: Control group, Propofol group, Propofol+miR-140-5p inhibitor group and miR-140-5p inhibitor group. Moreover, cell proliferation, apoptosis, migration and invasion of SGC-7901 and MKN45 cells were evaluated by BrdU incorporation assay, Annexin V-FITC/PI double staining assay, wound healing assay and transwell assay, respectively. The mRNA expressions of matrix metalloproteinase 2 (MMP-2) and MMP-9 were detected by qRT-PCR. Cleaved caspase-3, Bcl-2, MMP-2 and MMP-9 expressions were detected by Western blot.

Results: Propofol inhibited cell proliferation, migration and invasion, but promoted cell apoptosis in SGC-7901 and MKN45 cells. Propofol also elevated the expression of miR-140-5p. Suppression of miR-140-5p could reverse the effects of propofol on the biological behavior of SGC-7901 and MKN45 cells. Meanwhile, propofol treatment increased the expression of cleaved caspase-3 but decreased Bcl-2, MMP-2 and MMP-9 in SGC-7901 and MKN45 cells. The expression of cleaved caspase-3 was downregulated while Bcl-2, MMP-2 and MMP-9 were upregulated by miR-140-5p suppression.

Conclusion: Propofol could inhibit cell proliferation, migration and invasion, as well as promote cell apoptosis by upregulating miR-140-5p in gastric cancer cells.

Keywords: apoptosis; gastric cancer; miR-140-5p; migration; proliferation; propofol.

Conflict of interest statement

The authors declare that there is no conflict of interest regarding this work.

Figures

Figure 1
Figure 1
The effects of different concentrations of propofol on cell viability of MKN45 and SGC-7901 cells were measured by CCK-8 assay. *P < 0.05, vs. 0 μg/mL group. The values correspond to the mean ± standard deviation obtained from three independent experiments.
Figure 2
Figure 2
Relative miR-140-5p mRNA expression in MKN45 and SGC-7901 cells was detected by qRT-PCR. *P < 0.05, vs. Control group; #P < 0.05, vs. Propofol group, &P < 0.05, vs. miR-140-5p inhibitor group. The values correspond to the mean ± standard deviation obtained from three independent experiments.
Figure 3
Figure 3
miR-140-5p participated in the effects of propofol on MKN45 and SGC-7901 cell proliferation and apoptosis. (A) Cell proliferation was measured by BrdU incorporation assay (× 400). (B) Cell apoptosis was measured by Annexin V-FITC/PI double staining assay. (C) The protein expressions of cleaved caspase-3 and Bcl-2 were detected by Western blot. *P < 0.05, vs. Control group; #P < 0.05, vs. Propofol group, &P < 0.05, vs. miR-140-5p inhibitor group. The values correspond to the mean ± standard deviation obtained from three independent experiments.
Figure 4
Figure 4
miR-140-5p participated in the effects of propofol on MKN45 and SGC-7901 cell migration and invasion. (A) Cell migration ability was measured by Wound healing assay. (B) Cell migration ability was tested by Transwell assay (×200). (C) Cell invasion ability was detected by Transwell assay (×200). *P < 0.05, vs. Control group; #P < 0.05, vs. Propofol group, &P < 0.05, vs. miR-140-5p inhibitor group. The values correspond to the mean ± standard deviation obtained from three independent experiments.
Figure 5
Figure 5
Propofol suppressed the expression of MMP-2 and MMP-9 by upregulating miR-140-5p. (A) The mRNA expression of MMP-2 and MMP-9 were tested by qRT-PCR. (B and C) The protein expression levels of MMP-2 and MMP-9 were measured by Western blot. *P < 0.05, vs. Control group; #P < 0.05, vs. Propofol group, &P < 0.05, vs. miR-140-5p inhibitor group. The values correspond to the mean ± standard deviation obtained from three independent experiments.

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