High-throughput dense reconstruction of cell lineages

Open Biol. 2019 Dec;9(12):190229. doi: 10.1098/rsob.190229. Epub 2019 Dec 11.

Abstract

The first meeting exclusively dedicated to the 'High-throughput dense reconstruction of cell lineages' took place at Janelia Research Campus (Howard Hughes Medical Institute) from 14 to 18 April 2019. Organized by Tzumin Lee, Connie Cepko, Jorge Garcia-Marques and Isabel Espinosa-Medina, this meeting echoed the recent eruption of new tools that allow the reconstruction of lineages based on the phylogenetic analysis of DNA mutations induced during development. Combined with single-cell RNA sequencing, these tools promise to solve the lineage of complex model organisms at single-cell resolution. Here, we compile the conference consensus on the technological and computational challenges emerging from the use of the new strategies, as well as potential solutions.

Keywords: CRISPR/Cas9; dynamic barcoding; genetic switch; phylogenetic lineage reconstruction; scRNA-Seq.

Publication types

  • Consensus Development Conference
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CRISPR-Cas Systems
  • Cell Lineage / genetics*
  • Cell Tracking* / methods
  • Computational Biology
  • DNA Barcoding, Taxonomic
  • Gene Expression Profiling* / methods
  • High-Throughput Nucleotide Sequencing* / methods
  • Humans
  • Molecular Imaging* / methods
  • Mutation
  • Phylogeny
  • Single-Cell Analysis / methods