Formaldehyde (HCHO) is a reactive agent and the most essential common carcinogenic environmental pollutant. The present study investigated the protective and ameliorative effects of boric acid (BA) against formaldehyde-induced oxidative stress in A549 cell lines. The first group served as a control, the second group was treated with only 100 μM formaldehyde, and the third, fourth, and fifth groups were treated with 2.5, 5, and 10 mM BA, respectively. The sixth, seventh, and eighth groups were treated with 2.5, 5, and 10 mM BA plus 100 μM formaldehyde, respectively. In A549 cell lines, formaldehyde treatment significantly decreased cell viability, glutathione level, and enzyme activities of superoxide dismutase and catalase; however, malondialdehyde levels of the cell lysate were found to increase compared with the control group. In addition, formaldehyde treatment did not significantly alter nitric oxide levels. Meanwhile, mRNA expression levels of Tnf-α, NFĸB, and caspase-3 significantly increased but the Bcl-XL level did not show significant alteration by formaldehyde treatment. In contrast, the BA treatment reversed the formaldehyde-induced alteration in A549 cell lines. Consequently, BA exhibited a protective effect in A549 cell line against formaldehyde-induced lipid peroxidation. Furthermore, it ameliorated the antioxidant status and mRNA expression levels of proinflammatory cytokines.
Keywords: A549 cell line; Boric acid; Formaldehyde; Gene expression; Lipid peroxidation; Oxidative stress; Proinflammatory cytokines.