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, 11 (12), e1120-e1126
eCollection

Comparative Analysis of the Remineralization Potential of CPP-ACP With Fluoride, Tri-Calcium Phosphate and Nano Hydroxyapatite Using SEM/EDX - An in vitro Study

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Comparative Analysis of the Remineralization Potential of CPP-ACP With Fluoride, Tri-Calcium Phosphate and Nano Hydroxyapatite Using SEM/EDX - An in vitro Study

Charisma Thimmaiah et al. J Clin Exp Dent.

Abstract

Background: In recent years, the non-invasive management of non cavitated caries lesions using remineralization systems to repair the enamel have received more attention from the scientific community. Aim: To quantitatively evaluate the remineralization potential of Casein phosphopeptide-amorphous calcium phosphate-fluoride(CPP-ACPF), Tri-calcium phosphate(TCP) & Nano-hydroxyapatite(nHAP) using Scanning Electron Microscopy(SEM) and Energy dispersive X-ray Analysis(EDX).

Material and methods: 40 enamel specimens were prepared, and immersed in demineralising solution at a pH of 4.4 for 96 hours at 37°C, to induce artificial carious lesions. Remineralization was carried out for a period of 30 days using CPP-ACPF, TCP, nHAP. The specimens were evaluated for calcium and phosphorus content using SEM-EDX.

Results: The Ca/P mass % after remineralization was significantly higher with CPP-ACP-F and TCP-F followed by nHAP.

Conclusions: CPP-ACP-F and TCP can promote significant remineralization of incipient carious lesions. These are excellent delivery vehicles available in a slow release amorphous form to localize calcium, phosphate and fluoride at the tooth surface. Key words:Remineralization, in vitro; CPP-ACP fluoride, Nano-hydroxyapatite, Tri-calcium phosphate, SEM/ EDX.

Conflict of interest statement

Conflict of interest statement:The authors have declared that no conflict of interest exist.

Figures

Figure 1
Figure 1
Formula.
Figure 2
Figure 2
Consort diagram depicting the study protocol.
Figure 3
Figure 3
A) SEM image post demineralization of CPP-ACPF. B) EDX interpretation post demineralization of CPP-ACPF.
Figure 4
Figure 4
A) SEM image post remineralization of CPP-ACPF. B) EDX interpretation post remineralization of CPP-ACPF.

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