A fructan from Anemarrhena asphodeloides Bunge showing neuroprotective and immunoregulatory effects

Carbohydr Polym. 2020 Feb 1:229:115477. doi: 10.1016/j.carbpol.2019.115477. Epub 2019 Oct 17.


A novel polysaccharide, AAP70-1, was isolated from Anemarrhena asphodeloides for the first time. The primary structural analysis revealed that AAP70-1 was composed of glucose and fructose, had an absolute molecular weight of 2720 Da, and contained a (2→6)-linked β-D-fructofuranose (Fruf) backbone and a (2→1,6)-linked β-D-Fruf side chain with an internal α-D-glucopyranose (Glcp) in the form of a neokestose. To explore the potential factors responsible for the medicinally relevant bioactivities of A. asphodeloides, a biological assay was performed. Using flow cytometry analysis, AAP70-1 was experimentally shown to have neuroprotective effects, and it can prevent and ameliorate neurological damage via reducing apoptosis. The immunomodulation assay further revealed that AAP70-1 can significantly improve immune function by promoting phagocytic capacity and the secretion of cytokines (IL-6, IL-1β and TNF-α) in RAW264.7 cells. These results suggest that AAP70-1 has potential as a therapeutic agent for central nervous system diseases or as an immunomodulatory agent.

Keywords: Anemarrhena asphodeloides Bunge; Chloroform (PubChem CID: 6212); Cobalt dichloride (PubChem CID: 24288); D-Glucose (PubChem CID: 5793); Dimethyl sulfoxide (PubChem CID: 679); Fructan; Immunomodulation; Methyl iodide (PubChem CID: 6328); Neuroprotection; Neutral red (PubChem CID: 11105); Penicillin G (PubChem CID: 5904); Polysaccharide; Streptomycin (PubChem CID: 19649); Tocopherol (PubChem CID: 14986); Trifluoroacetic acid (PubChem CID: 6422).

MeSH terms

  • Anemarrhena / chemistry*
  • Animals
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Fructans / chemistry
  • Fructans / pharmacology*
  • Humans
  • Immunologic Factors / chemistry
  • Immunologic Factors / pharmacology*
  • Mice
  • Monosaccharides / analysis
  • Neuroprotective Agents / chemistry
  • Neuroprotective Agents / pharmacology*
  • Phagocytosis / drug effects
  • RAW 264.7 Cells
  • Structure-Activity Relationship


  • Fructans
  • Immunologic Factors
  • Monosaccharides
  • Neuroprotective Agents