Cell activity evaluation during bacterial bioluminescence oscillation

J Gen Appl Microbiol. 2020 Sep 30;66(4):201-206. doi: 10.2323/jgam.2019.07.002. Epub 2019 Dec 11.


Oscillation in bacterial bioluminescence from Photobacterium kishitanii liquid culture was examined regarding reproducibility and bacterial cell activities, i.e., dissolved oxygen (DO) consumption, esterase activity, and product production rate. A frequent increase in DO was suspected to be due to a rapid decrease in luminescence, and a simple model describing not only the monotonous decrease in cell activity, but also the luminescence-DO relationship is proposed.

Keywords: bioluminescent bacteria; dissolved oxygen; luminescence; oscillation; product.

MeSH terms

  • Biological Clocks*
  • Biomarkers / analysis
  • Esterases / metabolism*
  • Fluoresceins / analysis
  • Luminescence
  • Luminescent Measurements
  • Microbial Viability
  • Oxygen / metabolism*
  • Photobacterium / physiology*
  • Reproducibility of Results
  • Time Factors


  • Biomarkers
  • Fluoresceins
  • 5-carboxyfluorescein diacetate
  • Esterases
  • Oxygen

Supplementary concepts

  • Photobacterium kishitanii