Bacteriocin ASM1 is an O/S-diglycosylated, plasmid-encoded homologue of glycocin F

FEBS Lett. 2020 Apr;594(7):1196-1206. doi: 10.1002/1873-3468.13708. Epub 2019 Dec 25.


Here, we report on the biochemical characterization of a new glycosylated bacteriocin (glycocin), ASM1, produced by Lactobacillus plantarum A-1 and analysis of the A-1 bacteriocinogenic genes. ASM1 is 43 amino acids in length with Ser18-O- and Cys43-S-linked N-acetylglucosamine moieties that are essential for its inhibitory activity. Its only close homologue, glycocin F (GccF), has five amino acid substitutions all residing in the flexible C-terminal 'tail' and a lower IC50 (0.9 nm) compared to that of ASM1 (1.5 nm). Asm/gcc genes share the same organization (asmH← →asmABCDE→F), and the asm genes reside on an 11 905-bp plasmid dedicated to ASM1 production. The A-1 genome also harbors a gene encoding a 'rare' bactofencin-type bacteriocin. As more examples of prokaryote S-GlcNAcylation are discovered, the functions of this modification may be understood.

Keywords: S-linked glycopeptide bacteriostatic; bacteriocin; glycocin; plasmid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacteriocins / chemistry*
  • Bacteriocins / genetics
  • Bacteriocins / metabolism*
  • Base Sequence
  • Genes, Bacterial / genetics
  • Glycosylation
  • Lactobacillus plantarum / chemistry*
  • Lactobacillus plantarum / genetics*
  • Novobiocin
  • Phylogeny
  • Plasmids / genetics*
  • Sequence Analysis, DNA


  • Bacteriocins
  • glycocin F, Lactobacillus plantarum
  • plantaricin ASM1, Lactobacillus plantarum
  • Novobiocin

Associated data

  • GENBANK/KU896918
  • GENBANK/AB474371
  • GENBANK/GU552553
  • GENBANK/NZ_CP037439
  • GENBANK/AF304384
  • GENBANK/CP019716