Tensin1 expression and function in chronic obstructive pulmonary disease

Sci Rep. 2019 Dec 12;9(1):18942. doi: 10.1038/s41598-019-55405-2.

Abstract

Chronic obstructive pulmonary disease (COPD) constitutes a major cause of morbidity and mortality. Genome wide association studies have shown significant associations between airflow obstruction or COPD with a non-synonymous SNP in the TNS1 gene, which encodes tensin1. However, the expression, cellular distribution and function of tensin1 in human airway tissue and cells are unknown. We therefore examined these characteristics in tissue and cells from controls and people with COPD or asthma. Airway tissue was immunostained for tensin1. Tensin1 expression in cultured human airway smooth muscle cells (HASMCs) was evaluated using qRT-PCR, western blotting and immunofluorescent staining. siRNAs were used to downregulate tensin1 expression. Tensin1 expression was increased in the airway smooth muscle and lamina propria in COPD tissue, but not asthma, when compared to controls. Tensin1 was expressed in HASMCs and upregulated by TGFβ1. TGFβ1 and fibronectin increased the localisation of tensin1 to fibrillar adhesions. Tensin1 and α-smooth muscle actin (αSMA) were strongly co-localised, and tensin1 depletion in HASMCs attenuated both αSMA expression and contraction of collagen gels. In summary, tensin1 expression is increased in COPD airways, and may promote airway obstruction by enhancing the expression of contractile proteins and their localisation to stress fibres in HASMCs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins
  • Aged
  • Aged, 80 and over
  • Asthma / metabolism
  • Asthma / pathology
  • Humans
  • Immunohistochemistry
  • Lung / metabolism*
  • Lung / pathology
  • Middle Aged
  • Myocytes, Smooth Muscle / metabolism*
  • Myocytes, Smooth Muscle / pathology
  • Pulmonary Disease, Chronic Obstructive / metabolism*
  • Pulmonary Disease, Chronic Obstructive / pathology
  • Tensins / biosynthesis*
  • Transforming Growth Factor beta1 / metabolism
  • Up-Regulation

Substances

  • ACTA2 protein, human
  • Actins
  • TGFB1 protein, human
  • TNS1 protein, human
  • Tensins
  • Transforming Growth Factor beta1