Characterization of Nine Compounds Isolated From the Acid Hydrolysate of Lonicera fulvotomentosa Hsu Et S. C. Cheng and Evaluation of Their In Vitro Activity Towards HIV Protease

Molecules. 2019 Dec 11;24(24):4526. doi: 10.3390/molecules24244526.

Abstract

In this study, we isolated nine compounds from the acid hydrolysate of the flower buds of Lonicera fulvotomentosa Hsu et S. C. Cheng and characterized their chemical structures using 1H-NMR, 13C-NMR, and electron ionization mass spectroscopy (EI-MS). These compounds were identified as β-sitosterol (1), 5,5'-dibutoxy-2,2'-bifuran (2), nonacosane-10-ol (3), ethyl (3β)-3,23-dihydroxyolean-12-en-28-oate (4), oleanolic acid (5), ethyl caffeate (6), caffeic acid (7), isovanillin (8), and hederagenin (9), with 4 as a new triterpene compound. Inhibitory activity against human immunodeficiency virus (HIV) protease was also evaluated for the compounds, and only ethyl caffeate, caffeic acid, and isovanillin (6, 7, and 8) exhibited inhibitory effects, with IC50 values of 1.0 μM, 1.5 μM, and 3.5 μM, respectively. Molecular docking with energy minimization and subsequent molecular dynamic (MD) simulation showed that ethyl caffeate and caffeic acid bound to the active site of HIV protease, while isovanillin drifted out from the active site and dissociated into bulk water during MD simulations, and most of the binding residues of HIV protease have been previously identified for HIV protease inhibitors. These results suggest that caffeic acid derivatives may possess inhibitory activities towards HIV protease other than previously reported inhibitory activities against HIV integrase, and thus ethyl caffeate and caffeic acid could be used as lead compounds in developing potential HIV protease inhibitors, and possibly even dual-function inhibitors against HIV.

Keywords: HIV protease; Lonicera fulvotomentosa Hsu et S. C. Cheng; acid hydrolysate; inhibitor; molecular docking.

MeSH terms

  • Catalytic Domain
  • HIV Protease / chemistry
  • HIV Protease / metabolism*
  • HIV Protease Inhibitors / chemistry
  • HIV Protease Inhibitors / pharmacology*
  • HIV-1 / enzymology*
  • Lonicera / chemistry*
  • Mass Spectrometry
  • Molecular Docking Simulation
  • Molecular Dynamics Simulation
  • Molecular Structure
  • Phytochemicals / chemistry
  • Phytochemicals / pharmacology*
  • Plant Extracts / analysis

Substances

  • HIV Protease Inhibitors
  • Phytochemicals
  • Plant Extracts
  • HIV Protease