Culture of newt cells from different tissues and their expression of a regeneration-associated antigen

J Exp Zool. 1988 Jul;247(1):77-91. doi: 10.1002/jez.1402470111.


We have established culture conditions for cells from normal limb, early limb regenerate (blastema), heart, and liver of the newt Notophthalmus viridescens. Whereas heart and liver cells had a relatively short life in culture, limb cells have shown no sign of senescence over more than 1 year in culture. Cultured cells from all these tissues express to differing extents the regeneration-associated antigen 22/18. The antigen is intracellular and filamentous, and its expression appears to be regulated by culture density. Furthermore, 22/18 antigen is turned off in limb and blastemal cultures following differentiation into muscle, as also occurs in vivo.

MeSH terms

  • Animals
  • Antigens / analysis*
  • Cell Division
  • Cell Survival
  • Cells, Cultured
  • Extremities / cytology*
  • Extremities / physiology
  • Immunohistochemistry
  • Liver / cytology
  • Myocardium / cytology
  • Notophthalmus viridescens
  • Regeneration*


  • Antigens