Isolation and identification of cholestane and dihydropyrene from Calophyllum inophyllum

David Febrilliant Susanto; Hakun Wirawasista Aparamarta; Arief Widjaja; Nurul Jadid; Setiyo Gunawan

doi:10.1016/j.heliyon.2019.e02893

Isolation and identification of cholestane and dihydropyrene from Calophyllum inophyllum

Heliyon. 2019 Nov 21;5(11):e02893. doi: 10.1016/j.heliyon.2019.e02893. eCollection 2019 Nov.

Authors

David Febrilliant Susanto¹, Hakun Wirawasista Aparamarta¹, Arief Widjaja¹, Nurul Jadid², Setiyo Gunawan¹

Affiliations

¹ Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Surabaya, 60111, Indonesia.
² Department of Biology, Institut Teknologi Sepuluh Nopember, Surabaya, 60111, Indonesia.

Abstract

The highest population of Calophyllum inophyllum is in Indonesia. One of the bioactive compound contained in C. inophyllum leave is cholestane. The extraction method was employed to obtain crude extract from these leaves. It was followed by identification of the bioactive compounds. The purpose of this research was to develop a process for isolating and identifying cholestane and dihydropyrene from methanolic extract of C. inophyllum leaves in high yield and purity. The effect of crude extract to non-polar solvent mass ratio and non-polar solvent types on the separation of the bioactive compounds were also systematically investigated. New compounds (trans-2-[2-(trifluoromethyl)phenyl]-10b,10c-dimethyl-10b, 10c-dihydropyrene and anti-4-aza-B-homo-5.alpha-cholestane-3-one) were also identified in C. inophyllum leaves. The successful separation was obtained by employing CS₂ as the solvent and crude extract to CS₂ mass ratio of 1/10 (g/g).

Keywords: Biochemical engineering; Calophyllum inophyllum leaves; Chemical engineering; Extraction; Industrial chemistry; Materials characterization; Pharmaceutical engineering; Separation; Solvent; Transport process.