Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2020 Mar;103(3):e21652.
doi: 10.1002/arch.21652. Epub 2019 Dec 17.

Developing CRISPR-based Sex-Ratio Distorters for the Genetic Control of Fruit Fly Pests: A How to Manual

Affiliations
Review

Developing CRISPR-based Sex-Ratio Distorters for the Genetic Control of Fruit Fly Pests: A How to Manual

Konstantina T Tsoumani et al. Arch Insect Biochem Physiol. .

Abstract

Agricultural pest control using genetic-based methods provides a species-specific and environmentally harmless way for population suppression of fruit flies. One way to improve the efficiency of such methods is through self-limiting, female-eliminating approaches that can alter an insect populations' sex ratio toward males. In this microreview, we summarize recent advances in synthetic sex ratio distorters based on X-chromosome shredding that can induce male-biased progeny. We outline the basic principles to guide the efficient design of an X-shredding system in an XY heterogametic fruit fly species of interest using CRISPR/Cas gene editing, newly developed computational tools, and insect genetic engineering. We also discuss technical aspects and challenges associated with the efficient transferability of this technology in fruit fly pest populations, toward the potential use of this new class of genetic control approaches for pest management purposes.

Keywords: CRISPR/Cas9; Redkmer; X-chromosome; X-shredding system; spermatogenesis.

Similar articles

See all similar articles

References

REFERENCES

    1. Ant, T., Koukidou, M., Rempoulakis, P., Gong, H. F., Economopoulos, A., Vontas, J., & Alphey, L. (2012). Control of the olive fruit fly using genetics-enhanced sterile insect technique. BMC Biology, 10(1), 51.
    1. Aumann, R. A., Schetelig, M. F., & Häcker, I. (2018). Highly efficient genome editing by homology-directed repair using Cas9 protein in Ceratitis capitata. Insect Biochemistry and Molecular Biology, 101, 85-93.
    1. Bai, X., Zeng, T., Ni, X. Y, Su, H. A, Huang, J., Ye, G. Y, … Qi, Y. X (2019). CRISPR/Cas9-mediated knockout of the eye pigmentation gene white leads to alterations in color of head spots in the oriental fruit fly, Bactrocera dorsalis. Insect Molecular Biology, 28, 837-849.
    1. Burt, A. (2003). Site-specific selfish genes as tools for the control and genetic engineering of natural populations. Proceedings of the Royal Society of London. Series B: Biological Sciences, 270(1518), 921-928.
    1. Calkins, C., & Parker, A. (2005). Sterile Insect Quality, Sterile Insect Technique Principles and Practice in Area-Wide Integrated Pest Management (pp. 269-296). Springer.

LinkOut - more resources

Feedback