The InsP7 phosphatase Siw14 regulates inositol pyrophosphate levels to control localization of the general stress response transcription factor Msn2

J Biol Chem. 2020 Feb 14;295(7):2043-2056. doi: 10.1074/jbc.RA119.012148. Epub 2019 Dec 17.


The environmental stress response (ESR) is critical for cell survival. Yeast cells unable to synthesize inositol pyrophosphates (PP-InsPs) are unable to induce the ESR. We recently discovered a diphosphoinositol pentakisphosphate (PP-InsP5) phosphatase in Saccharomyces cerevisiae encoded by SIW14 Yeast strains deleted for SIW14 have increased levels of PP-InsPs. We hypothesized that strains with high inositol pyrophosphate levels will have an increased stress response. We examined the response of the siw14Δ mutant to heat shock, nutrient limitation, osmotic stress, and oxidative treatment using cell growth assays and found increased resistance to each. Transcriptional responses to oxidative and osmotic stresses were assessed using microarray and reverse transcriptase quantitative PCR. The ESR was partially induced in the siw14Δ mutant strain, consistent with the increased stress resistance, and the mutant strain further induced the ESR in response to oxidative and osmotic stresses. The levels of PP-InsPs increased in WT cells under oxidative stress but not under hyperosmotic stress, and they were high and unchanging in the mutant. Phosphatase activity of Siw14 was inhibited by oxidation that was reversible. To determine how altered PP-InsP levels affect the ESR, we performed epistasis experiments with mutations in rpd3 and msn2/4 combined with siw14Δ. We show that mutations in msn2Δ and msn4Δ, but not rpd3, are epistatic to siw14Δ by assessing growth under oxidative stress conditions and expression of CTT1 Msn2-GFP nuclear localization was increased in the siw14Δ. These data support a model in which the modulation of PP-InsPs influence the ESR through general stress response transcription factors Msn2/4.

Keywords: MSN2; SIW14; Saccharomyces cerevisiae; gene expression; inositol pyrophosphate; stress response; transcription regulation; yeast genetics.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cell Cycle / genetics
  • Cell Survival / genetics
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Diphosphates / metabolism
  • Gene Expression Regulation, Fungal / genetics
  • Inositol / metabolism
  • Osmotic Pressure / drug effects
  • Oxidation-Reduction
  • Oxidative Stress / genetics*
  • Peptides, Cyclic / genetics
  • Protein Tyrosine Phosphatases / genetics*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / genetics*
  • Saccharomyces cerevisiae Proteins / metabolism
  • Signal Transduction / genetics
  • Stress, Physiological / genetics*
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism


  • CTTHWGFTLC peptide
  • DNA-Binding Proteins
  • Diphosphates
  • MSN2 protein, S cerevisiae
  • Peptides, Cyclic
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • diphosphoric acid
  • Inositol
  • Protein Tyrosine Phosphatases
  • Siw14 protein, S cerevisiae