Enhanced mitochondrial membrane potential and ATP synthesis by photobiomodulation increases viability of the auditory cell line after gentamicin-induced intrinsic apoptosis

Sci Rep. 2019 Dec 17;9(1):19248. doi: 10.1038/s41598-019-55711-9.


Photobiomodulation (PBM) has been suggested to have a therapeutic effect on irreversible hearing loss induced by aminoglycosides, including gentamicin (GM). However, its intracellular mechanism(s) in GM-induced ototoxicity remain poorly understood. In the present study, we investigated the effect of PBM in GM-induced ototoxicity in auditory cells. We tried to characterize the downstream process by PBM, and the process that triggered the increased cell viability of auditory cells. As a result, the effects of PBM against GM-induced ototoxicity by increasing ATP levels and mitochondrial membrane potential was confirmed. These results suggest a theory to explain the therapeutic effects and support the use of PBM for aminoglycoside-induced hearing loss.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / biosynthesis*
  • Animals
  • Cell Line
  • Gentamicins / adverse effects*
  • Gentamicins / pharmacology
  • Hair Cells, Auditory* / metabolism
  • Hair Cells, Auditory* / pathology
  • Hearing Loss* / chemically induced
  • Hearing Loss* / metabolism
  • Hearing Loss* / pathology
  • Hearing Loss* / therapy
  • Low-Level Light Therapy*
  • Membrane Potential, Mitochondrial / drug effects*
  • Mice


  • Gentamicins
  • Adenosine Triphosphate