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, 12 (10), 1650-1656

Histological Assessment of the Efficiency of Rabbit Serum in Healing Skin Wounds

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Histological Assessment of the Efficiency of Rabbit Serum in Healing Skin Wounds

Abeer Ahmed Majeed et al. Vet World.

Abstract

Aim: This study aimed to investigate the impact of rabbit serum on skin wound healing with the help of histological examination.

Materials and methods: A total of ten indigenous rabbits were used in this study. The animals were divided into two groups: control and serum- treated. The histological assessment was done with a paraffin embedding technique and the histological sections were stained with H&E stain.

Results: Severe infiltration of polymorphonuclear leukocytes with severe fibrin deposits were seen in serum treated group at 2 days post-injury; at 7 days post-injury the changes revealed moderate fibroplasia, fibrin deposit and severe infiltration of both mononuclear and polymorphonuclear leukocytes; at 14 days post-injury, there were marked epithelization and dermal deposition of collagen fibers; and at 21 days post-injury, the epidermis completed epithelization and the dermis showed neither fibroplasia nor infiltration of mononuclear and polymorphonuclear leukocytes.

Conclusion: The results indicated that rabbit's serum can prevent wound infection, accelerate epithelialization and cutaneous regeneration with less granulation.

Keywords: healing; rabbits; serum; skin; wounds.

Figures

Figure-1
Figure-1
Section of skin (2 days post-injury in control group) shows clot (C), inflammatory zone (black arrows), edema (red arrows), and hemorrhage (H). Hematoxylin and eosin stain, 40×.
Figure-2
Figure-2
Magnified section of skin (2 days post-injury in control group) shows edema (E) and hemorrhage (arrows). Hematoxylin and eosin stain, 400×.
Figure-3
Figure-3
Section of skin (2 days post-injury in serum-treated group) shows mononuclear and polymorphonuclear leukocytes (arrows) and severe fibrin deposit (F). Hematoxylin and eosin stain, 400×.
Figure-4
Figure-4
Magnified section of skin (2 days post-injury in serum-treated group) shows clot (C), hemorrhage (H) with polymorphonuclear and mononuclear leukocytes (arrows). Hematoxylin and eosin stain, 400×.
Figure-5
Figure-5
Section of skin (7 days post-injury in control group) shows clot (C), inflammatory zone (In), mononuclear and polymorphonuclear leukocytes (arrows). Hematoxylin and eosin stain, 400×.
Figure-6
Figure-6
Section of skin (7 days post-injury in serum-treated group) shows clot (C), mononuclear and polymorphonuclear leukocytes (black arrows), inflammatory zone (In), fibrin deposited (F), and fibrogenesis (red arrows). Hematoxylin and eosin stain, 400×.
Figure-7
Figure-7
Section of skin (14 days post-injury in control group) shows clot (C), inflammatory zone (black arrows), and immature collagen fibers (G). Hematoxylin and eosin stain, 100×.
Figure-8
Figure-8
Section of skin (14 days post-injury in serum-treated group) shows epithelization (E) and deposit of immature collagen (arrows). Hematoxylin and eosin stain, 100×.
Figure-9
Figure-9
Section of skin (21 days post-injury in control group) shows thick epithelium (E) and immature granulation tissue (G). Hematoxylin and eosin stain, 100×.
Figure-10
Figure-10
Section of skin (21 days post-injury in serum-treated group) shows epithelium (E), fibrogenesis (F), and newly formed hair follicles (arrows). Hematoxylin and eosin stain, 100×.
Figure-11
Figure-11
Section of skin (28 days post-injury in control group) shows keratin layer (arrow), epithelization (E), and granulation tissue (G). Hematoxylin and eosin stain, 100×.
Figure-12
Figure-12
Section of skin (28 days post-injury in serum-treated group) shows keratin layer (red arrow), epithelium (E), dermis (D), and hair follicle (black arrows). Hematoxylin and eosin stain, 100×.

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