Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
, 7, 357
eCollection

Micro-Engineered Models of Development Using Induced Pluripotent Stem Cells

Affiliations
Review

Micro-Engineered Models of Development Using Induced Pluripotent Stem Cells

Pallavi Srivastava et al. Front Bioeng Biotechnol.

Abstract

During fetal development, embryonic cells are coaxed through a series of lineage choices which lead to the formation of the three germ layers and subsequently to all the cell types that are required to form an adult human body. Landmark cell fate decisions leading to symmetry breaking, establishment of the primitive streak and first tri-lineage differentiation happen after implantation, and therefore have been attributed to be a function of the embryo's spatiotemporal 3D environment. These mechanical and geometric cues induce a cascade of signaling pathways leading to cell differentiation and orientation. Due to the physiological, ethical, and legal limitations of accessing an intact human embryo for functional studies, multiple in-vitro models have been developed to try and recapitulate the key milestones of mammalian embryogenesis using mouse embryos, or mouse and human embryonic stem cells. More recently, the development of induced pluripotent stem cells represents a cell source which is being explored to prepare a developmental model, owing to their genetic and functional similarities to embryonic stem cells. Here we review the use of micro-engineered cell culture materials as platforms to define the physical and geometric contributions during the cell fate defining process and to study the underlying pathways. This information has applications in various biomedical contexts including tissue engineering, stem cell therapy, and organoid cultures for disease modeling.

Keywords: biomaterials; development; gastrulation models; iPS cells; micropatterning; morphogenesis.

Figures

Figure 1
Figure 1
(A) Graphical representation of effects of biomechanics and geometry in coordinating cell organization and germ-layer differentiation during embryonic development. (B) Various biochemical and biomechanical factors which have been documented to induce stem cell (MSC and PSCs) differentiation patterns in-vitro. Combining two or more of these factors for documenting cumulative cell responses is useful for understanding ECM based tissue modeling approaches.
Figure 2
Figure 2
(A) ES cells spatially organize themselves into three germ layers following BMP4 induction, depending on the size of the circle, creating a 2D gastrulation model. Image reused with permission (Deglincerti et al., 2016b) Nat Protoc 2016. (B) A semi-3D model of post-implantation amniotic sac embryoid (PASE)—The closest human model recapitulating multiple post-implantation embryogenic events. Image reused with permission (Shao et al., 2017) Nat Communications, 2017.

Similar articles

See all similar articles

References

    1. Abagnale G., Sechi A., Steger M., Zhou Q., Kuo C.-C., Aydin G., et al. . (2017). Surface topography guides morphology and spatial patterning of induced pluripotent stem cell colonies. Stem Cell Rep. 9, 654–666. 10.1016/j.stemcr.2017.06.016 - DOI - PMC - PubMed
    1. Abelseth E., Abelseth L., De la Vega L., Beyer S. T., Wadsworth S. J., Willerth S. M. (2019). 3D printing of neural tissues derived from human induced pluripotent stem cells using a fibrin-based bioink. ACS Biomater. Sci. Eng. 5, 234–243. 10.1021/acsbiomaterials.8b01235 - DOI
    1. Ahmed M., Ffrench-Constant C. (2016). Extracellular matrix regulation of stem cell behavior. Curr. Stem Cell Rep. 2, 197–206. 10.1007/s40778-016-0056-2 - DOI - PMC - PubMed
    1. Alakpa E. V., Jayawarna V., Lampel A., Burgess K. V., West C. C., Bakker S. C. J., et al. (2016). Tunable supramolecular hydrogels for selection of lineage-guiding metabolites in stem cell cultures. Chem 1, 298–319. 10.1016/j.chempr.2016.07.001 - DOI
    1. Alfandari D., McCusker C., Cousin H. (2009). ADAM function in embryogenesis. Semin. Cell Dev. Biol. 20, 153–163. 10.1016/j.semcdb.2008.09.006 - DOI - PMC - PubMed

LinkOut - more resources

Feedback