The Arabidopsis Transcriptome Responds Specifically and Dynamically to High Light Stress

Cell Rep. 2019 Dec 17;29(12):4186-4199.e3. doi: 10.1016/j.celrep.2019.11.051.

Abstract

The dynamic and specific transcriptome for high light (HL) stress in plants is poorly understood because heat has confounded previous studies. Here, we perform an in-depth temporal responsive transcriptome analysis and identify the core HL-responsive genes. By eliminating the effect of heat, we uncover a set of genes specifically regulated by high-intensity light-driven signaling. We find that 79% of HL-responsive genes restore their expression to baseline within a 14-h recovery period. Our study reveals that plants respond to HL through dynamic regulation of hormones, particularly abscisic acid (ABA), photosynthesis, and phenylpropanoid pathway genes. Blue/UV-A photoreceptors and phytochrome-interacting factor (PIF) genes are also responsive to HL. We further show that ABA biosynthesis-defective mutant nced3nced5, as well as pif4, pif5, pif4,5, and pif1,3,4,5 mutants, are hypersensitive to HL. Our study presents the dynamic and specific high-intensity light-driven transcriptional landscape in plants during HL stress.

Keywords: ABA; Arabidopsis; BBX; PIF; SAUR; high light; time course; transcriptome.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics*
  • Arabidopsis / radiation effects*
  • Arabidopsis Proteins / genetics*
  • Gene Expression Regulation, Plant / genetics
  • Gene Expression Regulation, Plant / radiation effects
  • Light*
  • Transcriptome / genetics*
  • Transcriptome / radiation effects

Substances

  • Arabidopsis Proteins