Type 2 diabetes mellitus (T2DM) is an important reason to cause chronic wound healing or even amputation to patients. A common characteristic of T2DM is the presence of hyperglycemia. Autophagy, a cellular pathway which related to protein and organelle degradation, is relevant to many types of cellular homeostasis change and human diseases including diabetes. However, the relationship between high glucose and autophagy in keratinocytes remains unclear. Previously in our research, wnt7a was proved to accelerate healing process by promoting angiogenesis and ameliorating local inflammation, while little is known about its role in epithelial cells, namely keratinocytes. We hypothesized that reduced expression of wnt7a may contribute to the increment of autophagy. We then compared the expression of autophagic markers such as nlrp3, LC3A/B and bip by high glucose-cultured HaCaT cells, with or without wnt7a treatment. Then we examined the expression levels of TNF-α, TLR-4 and p62 to assess inflammatory statement. High glucose induced a significant increasement in the expression of both autophagic markers and inflammatory markers and these elevated protein levels were reversed after the use of wnt7a. Therefore, these results showed that wnt7a regulates overwhelmed autophagy and inflammation promoted under high glucose condition.
Keywords: Autophagy; Inflammation; Keratinocytes; Wnt7a.
Copyright © 2019. Published by Elsevier Ltd.