Human muscle-derived CLEC14A-positive cells regenerate muscle independent of PAX7

Nat Commun. 2019 Dec 18;10(1):5776. doi: 10.1038/s41467-019-13650-z.


Skeletal muscle stem cells, called satellite cells and defined by the transcription factor PAX7, are responsible for postnatal muscle growth, homeostasis and regeneration. Attempts to utilize the regenerative potential of muscle stem cells for therapeutic purposes so far failed. We previously established the existence of human PAX7-positive cell colonies with high regenerative potential. We now identified PAX7-negative human muscle-derived cell colonies also positive for the myogenic markers desmin and MYF5. These include cells from a patient with a homozygous PAX7 c.86-1G > A mutation (PAX7null). Single cell and bulk transcriptome analysis show high intra- and inter-donor heterogeneity and reveal the endothelial cell marker CLEC14A to be highly expressed in PAX7null cells. All PAX7-negative cell populations, including PAX7null, form myofibers after transplantation into mice, and regenerate muscle after reinjury. Transplanted PAX7neg cells repopulate the satellite cell niche where they re-express PAX7, or, strikingly, CLEC14A. In conclusion, transplanted human cells do not depend on PAX7 for muscle regeneration.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

MeSH terms

  • Animals
  • Biopsy
  • Cell Adhesion Molecules / physiology*
  • Child, Preschool
  • Consanguinity
  • Exome Sequencing
  • Female
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Lectins, C-Type / physiology*
  • Male
  • Mice
  • Muscle, Skeletal / cytology
  • Muscle, Skeletal / injuries
  • Muscle, Skeletal / physiology*
  • Mutation
  • PAX7 Transcription Factor / genetics*
  • PAX7 Transcription Factor / metabolism
  • Primary Cell Culture
  • Regeneration*
  • Satellite Cells, Skeletal Muscle / physiology*
  • Satellite Cells, Skeletal Muscle / transplantation
  • Single-Cell Analysis
  • Transplantation, Heterologous / methods
  • Wasting Syndrome / genetics*
  • Wasting Syndrome / therapy


  • CLEC14A protein, human
  • Cell Adhesion Molecules
  • Lectins, C-Type
  • PAX7 Transcription Factor
  • PAX7 protein, human